Mouse ESAM Gene cDNA Clone / ORF Clone

ESAM cDNA Clone Datasheet

ESAM cDNA Clone Product Information

• Gene Bank Ref.ID: NM_027102.3
• cDNA Size: 1185
• Gene Synonym: Esam1; W117m; 2310008D05Rik; Esam
• Source: Mouse

ESAM cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus endothelial cell-specific adhesion molecule DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

ESAM cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

ESAM cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

ESAM cDNA Clone Sequence and Quality Control

Sequence:

1        ATGATTCTTC AGGCTGGAAC CCCCGAGACC AGCTTGCTGC GGGTTTTGTT CCTGGGACTG
61       AGTACCCTTG CTGCCTTCTC CCGAGCTCAG ATGGAGTTGC ACGTGCCCCC GGGCCTCAAC
121      AAATTGGAAG CGGTAGAGGG AGAAGAAGTG GTGCTCCCCG CCTGGTACAC GATGGCACGG
181      GAGGAGTCGT GGTCCCACCC CCGGGAGGTG CCCATCCTGA TCTGGTTCTT GGAACAAGAA
241      GGGAAGGAAC CAAACCAGGT GTTGTCTTAC ATTAATGGAG TCATGACAAA TAAACCTGGA
301      ACAGCCCTGG TCCACTCTAT CTCTTCACGG AATGTGTCCC TGCGCCTGGG GGCACTCCAG
361      GAGGGAGACT CTGGGACTTA CCGCTGTTCT GTCAATGTGC AGAATGATGA AGGCAAAAGT
421      ATAGGCCACA GCATCAAAAG CATAGAGCTC AAAGTGCTGG TTCCTCCAGC TCCTCCATCC
481      TGTAGTTTAC AGGGTGTACC CTATGTCGGG ACCAATGTGA CCCTGAACTG CAAGTCCCCA
541      AGGAGTAAAC CTACTGCTCA GTACCAGTGG GAGAGGCTGG CCCCATCCTC CCAGGTCTTC
601      TTTGGACCAG CCTTAGATGC TGTTCGTGGA TCTTTAAAGC TCACTAACCT TTCCATTGCC
661      ATGTCTGGAG TCTATGTCTG CAAGGCTCAA AACAGAGTGG GCTTTGCCAA GTGCAACGTG
721      ACCTTGGACG TGATGACAGG GTCCAAGGCT GCAGTGGTCG CTGGAGCAGT TGTGGGCACT
781      TTTGTTGGGT TGGTGCTGAT AGCTGGGCTG GTCCTGTTGT ACCAGCGCCG GAGCAAGACC
841      TTGGAAGAGC TGGCCAATGA TATCAAGGAA GATGCCATTG CTCCCCGGAC CTTGCCTTGG
901      ACCAAAGGCT CAGACACAAT CTCCAAGAAT GGGACACTTT CTTCGGTCAC CTCAGCACGA
961      GCTCTGCGGC CACCCAAGGC TGCTCCTCCA AGACCTGGCA CATTTACTCC CACACCCAGT
1021     GTCTCTAGCC AGGCCCTGTC CTCACCAAGA CTGCCCAGGG TAGATGAACC CCCACCTCAG
1081     GCAGTGTCCC TGACCCCAGG TGGGGTTTCT TCTTCTGCTC TGAGCCGCAT GGGTGCTGTG
1141     CCTGTGATGG TGCCTGCACA GAGTCAGGCT GGGTCTCTTG TGTGA

 

Quality Control:cDNA sequencing results is available upon request.