Mouse ESAM Protein (His Tag)

Endothelial cell-specific adhesion molecule Protein Datasheet

ESAM Protein Product Information

• Synonym: 2310008D05Rik, Esam1, W117m
• Protein Construction: A DNA sequence encoding the mouse ESAM (Q925F2) (Met 1-Ala 251) was expressed, with a C-terminal polyhistidine tag.
• Source: Mouse
• Expression Host: Human Cells

ESAM Protein QC Testing

• Purity: > 92 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Gln 30
• Molecular Mass: The secreted recombinant mouse ESAM comprises 233 amino acids and has a calculated molecular mass of 25.6 kDa. As a result of different glycosylation, the apparent molecular mass of the recombinant protein is approximately 33-43 kDa in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: ESAM protein

ESAM Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

ESAM Protein Description

Endothelial cell-selective adhesion molecule (ESAM) is a member of JAM family of immunoglobulin superfamily and consists of one V-type and one C2-type immunoglobulin domain, as well as a hydrophobic signal sequence, a single transmembrane region, and a cytoplasmic domain. ESAM is specifically expressed at endothelial tight junctions and on activated platelets and performs homophilic adhesion activity. The adaptor protein membrane-associated guanylate kinase MAGI-1 has been identified as an intracellular binding partner of ESAM. In addition, ESAM at endothelial tight junctions participates in the migration of neutrophils through the vessel wall, possibly by influencing endothelial cell contacts. ESAM-deficient mice were described with lowered angiogenic potential, and accordingly, overexpression of ESAM is closely associated with certain tumor growth and metastasis.

References

1. Hirata Ki. et al., 2001, J Biol Chem. 276: 16223-31.
2. Ishida T. et al., 2003, J Biol Chem. 278: 34598-604.
3. Wegmann F. et al., 2006, J Exp Med. 203: 1671-7.
4. Wegmann F. et al., 2004, Exp Cell Res. 300: 121-33.
5. Clasper S. et al., 2008, Cancer Res. 68: 7293-303.