ESAM Antibody

ESAM Antibody Datasheet

ESAM Antibody Product Information

• Immunogen: Recombinant Human ESAM protein (Catalog#10187-H08H)
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 202
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Human ESAM (rh ESAM; Catalog#10187-H08H; NP_620411.2; Met 1-Ala 248).

ESAM Antibody Usage Guide

• Specificity: Human ESAM
• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human ESAM in WB. Using a DAB detection system, the detection limit for Human ESAM is approximately 1 ng/lane under non-reducing conditions and reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human ESAM. The detection limit for Human ESAM is approximately 0.15625 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

ESAM Antibody Background

Endothelial cell-selective adhesion molecule (ESAM) is a member of JAM family of immunoglobulin superfamily and consists of one V-type and one C2-type immunoglobulin domain, as well as a hydrophobic signal sequence, a single transmembrane region, and a cytoplasmic domain. ESAM is specifically expressed at endothelial tight junctions and on activated platelets and performs homophilic adhesion activity. The adaptor protein membrane-associated guanylate kinase MAGI-1 has been identified as an intracellular binding partner of ESAM. In addition, ESAM at endothelial tight junctions participates in the migration of neutrophils through the vessel wall, possibly by influencing endothelial cell contacts. ESAM-deficient mice were described with lowered angiogenic potential, and accordingly, overexpression of ESAM is closely associated with certain tumor growth and metastasis.

References

1. Hirata, Ki. et al., 2001, J. Biol. Chem. 276: 16223-16231.
2. Ishida, T. et al., 2003, J. Biol. Chem. 278: 34598-34604.
3. Wegmann, F. et al., 2006, J. Exp. Med. 203: 1671-1677.
4. Wegmann, F. et al., 2004, Exp. Cell. Res. 300: 121-133.
5. Clasper, S. et al., 2008, Cancer. Res. 68: 7293-7303.