ERP72 / PDIA4 Protein

Protein disulfide isomerase family A, member 4 Protein Datasheet

ERP72 / PDIA4 Protein Product Information

• Synonym: PDIA4, ERP70, ERP72
• Protein Construction: A DNA sequence encoding the human PDIA4 (P13667)(Met1-Thr641) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
• Source: Human
• Expression Host: Human Cells

ERP72 / PDIA4 Protein QC Testing

• Purity: > 90 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Val 21
• Molecular Mass: The recombinant human PDIA4/Fc is a disulfide-linked homodimer. The reduced monomer comprises 862 amino acids and has a predicted molecular mass of 97.2 kDa. The apparent molecular mass of the protein is approximately 96 kDa in SDS-PAGE under reducing conditions
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: ERP72 / PDIA4 protein

ERP72 / PDIA4 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

ERP72 / PDIA4 Protein Description

ERP72, also known as PDIA4, is an endoplasmic reticulum luminal protein.  It is a member of the protein disulfide isomerase family. As a stress protein, ERP72 participates in the catalysis of protein-S-S-bond rearrangement. Both of PDIA4 and PDIA3 function as proteases, protein disulfide isomerases, phospholipases or an arrangement of these. ERP72 also compose part of a large chaperone multiprotein complex comprising CABP1, DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX.

References

1. Tsai YC. et al., 2012, Mol Cell Proteomics. 11 (5): 60-76.
2. Kim W. et al., 2011, Mol Cell. 44 (2): 325-40
3. Vinayagam A. et al., 2011, Sci Signal. 4 (189): 8.