ERN1 Antibody

ERN1 Antibody Datasheet

ERN1 Antibody Product Information

• Immunogen: Recombinant Human ERN1 protein (Catalog#11905-H20B)
• Antibody Type: Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
• Clone ID: 10B2B2
• Ig Type: Mouse IgG2b
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human ERN1 (rh ERN1; Catalog#11905-H20B; O75460-1; Pro 465-Leu 977). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

ERN1 Antibody Usage Guide

• Specificity: Human ERN1
• No cross-reactivity in ELISA with

  Baculovirus lysate

• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human ERN1 in WB. Using a DAB detection system, the detection limit for Human ERN1 is approximately 4 ng/lane under non-reducing conditions and reducing conditions.
• Direct ELISA: This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human ERN1. The detection limit for Human ERN1 is approximately 0.31 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

ERN1 Antibody Background

Serine / threonine-protein kinase/endoribonuclease IRE1, also known as, endoplasmic reticulum-to-nucleus signaling 1, inositol-requiring protein 1, Ire1-alpha, ERN1 and IRE1, is a single-pass type I membrane protein which belongs to theprotein kinase superfamily and Ser/Thr protein kinase family. ERN1 contains oneKEN domain and oneprotein kinase domain. ERN1 is ubiquitously expressed. High levels of ERN1 is observed in pancreatic tissue. ERN1 binds HSPA5, a negative regulator of the unfolded protein response. This interaction may disrupt homodimerization and prevent activation of ERN1. ERN1 also interacts with TAOK3 and TRAF2. ERN1 senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain of ERN1 splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.The kinase domain of ERN1 is activated by trans-autophosphorylation. Kinase activity of ERN1 is required for activation of the endoribonuclease domain.

References

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3. Liu C.Y., et al., 2003, J. Biol. Chem. 278: 17680-7.
4. Oppermann F.S., et al., 2009, Mol. Cell. Proteomics 8: 1751-64.