Human ERMAP Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13423-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

ERMAP cDNA Clone Datasheet

ERMAP cDNA Clone Product Information

Gene Bank Ref.ID:	NM_018538.3
cDNA Size:	1428
Gene Synonym:	RD; SC; PRO2801; ERMAP
Source:	Human

ERMAP cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens erythroblast membrane-associated protein (Scianna blood group) DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

ERMAP cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

ERMAP cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

ERMAP cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGAGATGG CGAGTTCTGC TGGCTCCTGG CTCTCTGGCT GCCTCATCCC TCTCGTCTTC
61       CTCCGGCTGT CTGTGCATGT GTCAGGCCAC GCAGGGGATG CCGGCAAGTT CCACGTGGCC
121      CTACTAGGGG GCACAGCCGA GCTGCTCTGC CCTCTCTCCC TCTGGCCCGG GACGGTACCC
181      AAGGAGGTGA GGTGGCTGCG GTCCCCATTC CCGCAGCGCT CCCAGGCTGT TCACATATTC
241      CGGGATGGGA AGGACCAGGA TGAAGATCTG ATGCCGGAAT ATAAGGGGAG GACGGTGCTA
301      GTGAGAGATG CCCAAGAGGG AAGTGTCACT CTGCAGATCC TTGACGTGCG CCTTGAGGAC
361      CAAGGGTCTT ACCGATGTCT GATCCAAGTT GGAAATCTGA GTAAAGAGGA CACCGTGATC
421      CTGCAGGTTG CAGCCCCATC TGTGGGGAGT CTCTCCCCCT CAGCAGTGGC TCTGGCTGTG
481      ATCCTGCCTG TCCTGGTACT TCTCATCATG GTGTGCCTTT GCCTTATCTG GAAGCAAAGA
541      AGAGCAAAAG AAAAGCTTCT CTATGAACAT GTGACGGAGG TGGACAATCT TCTTTCAGAC
601      CATGCTAAAG AAAAAGGAAA ACTCCATAAA GCTGTCAAGA AACTCCGGAG TGAACTGAAG
661      TTGAAAAGAG CTGCAGCAAA CTCAGGCTGG AGAAGAGCCC GGTTGCATTT TGTGGCAGTG
721      ACCCTGGACC CAGACACAGC ACATCCCAAA CTCATCCTTT CTGAGGACCA AAGATGTGTA
781      AGGCTTGGAG ACAGACGGCA GCCTGTACCT GACAACCCCC AGAGATTTGA TTTCGTTGTC
841      AGCATCCTAG GCTCTGAGTA CTTCACGACT GGCTGCCACT ACTGGGAGGT GTATGTGGGA
901      GACAAGACCA AATGGATTCT TGGAGTATGT AGTGAGTCAG TGAGCAGGAA GGGGAAGGTT
961      ACTGCCTCAC CTGCCAATGG ACACTGGCTT CTGCGACAGA GTCGTGGGAA TGAGTATGAA
1021     GCTCTCACAT CCCCGCAGAC CTCCTTCCGC CTTAAAGAGC CTCCACGGTG TGTGGGGATT
1081     TTCCTGGACT ATGAAGCAGG AGTCATCTCT TTCTACAATG TGACCAACAA GTCCCACATC
1141     TTTACTTTCA CCCACAATTT CTCTGGCCCC CTTCGCCCTT TCTTTGAACC TTGCCTTCAT
1201     GATGGAGGAA AAAACACAGC ACCTCTAGTC ATTTGTTCAG AACTACACAA ATCAGAGGAA
1261     TCAATTGTCC CCAGGCCAGA AGGGAAAGGC CATGCTAATG GAGATGTGTC CCTCAAGGTG
1321     AACTCTTCTT TACTACCCCC GAAGGCCCCA GAGCTGAAGG ATATAATCCT GTCCTTGCCC
1381     CCTGACCTTG GCCCAGCCCT TCAGGAGCTC AAGGCTCCTT CTTTTTAG

Quality Control:cDNA sequencing results is available upon request.