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Rabbit ERBB3/HER3 Gene ORF cDNA clone in cloning vector

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    Rabbit ErbB3/HER3 cDNA Clone Product Information
    NCBI RefSeq:XM_002711067.1
    RefSeq ORF Size:4026bp
    cDNA Description:Full length Clone DNA of Rabbit v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian).
    Gene Synonym:ERBB3
    Species:Rabbit
    Vector:pGEM-T Vector
    Plasmid:pGEM-rabERBB3
    Restriction Site:
    Tag Sequence:
    Sequence Description:Identical with the Gene Bank Ref. ID sequence except for the point mutations: 1169 G/A resulting in the amino acid arg substitution by his and 966 C/T,1281 C/T,1473 G/A,1557 C/T,1671 A/G not causing the amino acid variation.
    Sequencing primers:SP6 and T7 or M13-47 and RV-M
    Promoter:
    Application:
    Antibiotic in E.coli:Ampicillin
    Antibiotic in mammalian cell:
    Shipping_carrier:Each tube contains lyophilized plasmid.
    Storage:The lyophilized plasmid can be stored at room temperature for three months.
    Flow cytometric analysis of Human CD32 expression on human whole blood lymphocytes (left panel), monocytes (middle panel) and granulocytes (right panel). Cells were stained with purified anti-Human CD32, then a FITC-conjugated second step antibody. The histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes, monocytes and granulocytes.
    pGEM-T Vector Information

    The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

    pGEM-T Simple Usage Suggestion:

    The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

    Vector Sequence Download
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    Background

    ErbB3, also known as Her3(human epidermal growth factor receptor3), is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound glycoprotein has a neuregulin binding domain but has not an active kinase domain., and therefore can not mediate the intracellular signal transduction through protein phosphorylation. However, its heterodimer with ErbB2 or other EGFR members responsible for tyrosine phosphorylation forms a receptor complex with high affinity, and initiates the related pathway which lead to cell proliferation or differentiation. ErbB3 has been shown to implicated in numerous cancers, including prostate, bladder, and breast tumors. This protein has different isoforms derived from alternative splicing variants, and among which, the secreted isoform lacking the intermembrane region modulates the activity of membrane-bound form.

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    Catalog: TG65001-G
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