Mouse ERBB3 partial cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
MG51003-C	Vector 3 ($295) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

ERBB3 partical cDNA Clone Datasheet

ERBB3 partical cDNA Clone Product Information

cDNA Size:	1975
Gene Synonym:	Her3; C76256; Erbb-3; Erbb3r
Source:	Mouse

ERBB3 partical cDNA Clone Description

cDNA Description:

ORF Clone of Mus musculus v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian) DNA.

This cDNA clone has gone through customized codon optimization in order to obtain high level of protein expression in particular cell lines. Therefore, although the translated amino acid sequence is identical to the amino sequence on Gene Bank, the DNA sequence is different from that on Gene Bank.

Vector:

Vector 3. Restriction site: Hind III + XbaI

Optional Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

His Tag Sequence:

CATCACCACCATCACCACCATCATCACCAT

ERBB3 partical cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

ERBB3 partical cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

ERBB3 partical cDNA Clone Sequence and Quality Control

Sequence:

This sequece is the same as ERBB3 partical

1        AAGCTTACCA TGAGTGCCAT TGGAACCCTG CAAGTGCTGG GCTTCCTGCT CAGCCTGGCA
61       CGGGGATCTG AGATGGGCAA CTCACAGGCA GTGTGCCCTG GCACCCTGAA TGGCCTGAGT
121      GTGACAGGGG ATGCTGACAA CCAGTACCAG ACCCTGTACA AGCTCTATGA GAAGTGTGAG
181      GTGGTCATGG GCAACCTGGA AATTGTGCTG ACTGGACACA ATGCGGACCT GAGCTTCCTG
241      CAGTGGATCA GGGAGGTGAC AGGCTATGTG CTGGTGGCCA TGAATGAGTT CTCTGTCCTG
301      CCCCTGCCCA ACCTCCGGGT GGTGAGAGGT ACCCAGGTGT ATGATGGGAA GTTTGCCATC
361      TTTGTCATGC TGAACTACAA CACCAACTCC AGCCATGCCC TGCGCCAGCT CCGGTTCACC
421      CAACTGACTG AGATCCTGCT GGGAGGGGTG TACATTGAAA AGAATGACAA ACTGTGCCAC
481      ATGGACACCA TTGACTGGAG GGACATTGTG CGGGTGCCAG ATGCAGAGAT TGTGGTCAAG
541      AACAATGGGG GTAACTGCCC TCCCTGCCAT GAGGTGTGCA AGGGTCGCTG CTGGGGACCT
601      GGACCAGAGG ACTGCCAGAT CCTGACCAAG ACCATCTGTG CCCCACAGTG CAATGGTCGC
661      TGCTTTGGAC CCAACCCCAA CCAGTGCTGC CATGATGAAT GTGCTGGTGG CTGCAGTGGA
721      CCCCAGGACA CAGACTGCTT TGCCTGCCGC CACTTCAATG ACTCTGGAGC CTGTGTGCCC
781      CGCTGCCCTG CCCCACTGGT GTACAACAAG CTGACCTTCC AACTGGAGCC CAACCCTCAC
841      ATCAAATACC AGTATGGTGG AGTCTGTGTG GCTTCCTGCC CCCATAACTT TGTGGTGGAC
901      CAGACCTTCT GTGTCAGGGC CTGCCCAGCA GACAAGATGG AGGTGGACAA GAATGGCCTC
961      AAGATGTGTG AGCCCTGCCG AGGACTGTGT CCCAAGGCAT GTGAAGGCAC TGGCAGCGGA
1021     TCCCGCTACC AGACAGTGGA CAGCTCCAAC ATTGATGGCT TTGTGAACTG CACCAAGATC
1081     CTGGGCAACC TGGACTTCCT GATCACTGGC CTCAATGGGG ACCCCTGGCA CAAAATCCCT
1141     GCCCTGGACC CAGAGAAGCT CAATGTCTTC CGCACAGTTC GGGAGATCAC TGGCTACCTG
1201     AACATCCAGA GCTGGCCCCC TCACATGCAC AACTTCTCTG TGTTCTCCAA CCTGACCACC
1261     ATTGGGGGAA GAAGCCTCTA CAACAGGGGT TTCTCCCTGC TGATCATGAA GAACCTGAAT
1321     GTGACCAGCC TGGGATTCCG CTCCCTGAAG GAAATCAGTG CTGGAAGAGT CTACATCTCT
1381     GCCAATCAGC AGCTGTGCTA CCACCACTCC CTGAACTGGA CACGCCTGCT GCGGGGACCT
1441     GCTGAAGAGC GCCTGGACAT CAAATACAAC CGCCCTCTGG GGGAGTGTGT GGCAGAGGGC
1501     AAGGTGTGTG ACCCACTGTG CAGTAGCGGA GGATGCTGGG GTCCAGGACC TGGCCAGTGC
1561     CTGTCATGCC GCAACTACAG CAGGGAAGGT GTGTGTGTCA CCCACTGTAA TGTGCTGCAG
1621     GGGGAGCCAC GAGAGTTTGT GCATGAGGCC CACTGCTTCT CCTGCCACCC TGAATGCCAG
1681     CCAATGGAGG GAACCAGCAC CTGCAATGGC TCTGGCAGTG ATGCCTGTGC CCGCTGTGCC
1741     CACTTCAGGG ATGGTCCCCA CTGTGTGAAC TCCTGCCCAC ATGGTATCCT GGGAGCCAAG
1801     GGACCTATCT ACAAGTACCC AGATGCCCAG AATGAGTGCC GACCATGCCA TGAGAACTGC
1861     ACCCAAGGCT GCAAGGGACC AGAACTTCAG GACTGTCTGG GACAGGCAGA GGTCCTGATG
1921     AGCAAACCCC ACGCT¹CATCA CCACCATCAC CACCATCATC ACCAT²TAAAT CTAGA

1. GCT was the nucleotide residue from the restriction site during plasmid construction,
which has no influence on protein expression.
2. 10 × His Tag

Quality Control:cDNA sequencing results is available upon request.