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Canine ERBB2 Gene cDNA Clone / ORF Clone
| Catalog | Vector (Price) | Quantity | In Stock | Operation |
| DG70024-G |
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YES |  |
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* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. |
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ERBB2 cDNA Clone Datasheet
ERBB2 cDNA Clone Product Information
| Gene Bank Ref.ID: | EU239096.1 |
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cDNA Size: |
3783 |
| Gene Synonym: |
HER-2; ERBB2 |
| Source: | Canine |
ERBB2 cDNA Clone Description
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cDNA Description: |
ORF Clone of Canis lupus familiaris v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) DNA. |
| Vector: | Cloning Vector: pGEM-T Vector
Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG |
| Sequence Description: | Same as Gene Bank Ref. ID sequence with the nucleotide (1-39) insertion mutation , the point mutation 2225 T/C resulting in the amino acid Val substitution by Ala and 1587 G/A，1689 T/C not causing the amino acid variation. |
ERBB2 cDNA Clone Shipping
- 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
- It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.
ERBB2 cDNA Clone Usage Protocol
- Cut along the circle edge of the spot (arrow points to) to get the filter paper.
- Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O Ensure the filter is completely immersed in the H2O by briefly centrifuging the tube for 10 seconds.
- Transfer the tube to incubation at 95℃ for 15-30 min.
- At the end of the incubation pulse vortex, or gently tap the tube.
- Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.
ERBB2 cDNA Clone Sequence and Quality Control
Sequence:
1 ATGGAGCTGG CGGCCTGGTG CCGCTGGGGG CTCCTTCTCG CCCTCCTGCC CTCCGGAGCC 61 GCGGGCACCC AAGTGTGCAC CGGCACAGAC ATGAAGCTCC GGCTCCCGGC CAGTCCCGAG 121 ACCCACCTGG ATATGCTCCG CCACCTGTAC CAGGGCTGTC AAGTGGTACA GGGGAACCTG 181 GAGCTCACTT ACCTGCCTGC CAATGCCAGC CTGTCCTTCC TGCAGGATAT CCAGGAGGTG 241 CAGGGCTATG TGCTCATTGC TCACAGCCAA GTGAGGCAGA TCCCACTGCA GAGGCTACGA 301 ATTGTGCGAG GCACCCAGCT CTTTGAGGAC AACTACGCCC TGGCCGTGCT GGACAATGGA 361 GACCCGCTGG AGGGTGGCAT CCCTGCACCA GGGGCGGCCC CAGGAGGGCT GCGGGAGCTG 421 CAGCTTCGAA GCCTCACAGA GATCCTGAAG GGAGGGGTCT TGATTCAGCG GAGCCCGCAG 481 CTCTGCCACC AGGACACGAT TTTATGGAAG GACGTCTTCC ATAAGAACAA CCAGCTGGCC 541 CTCACGCTGA TAGACACCAA CCGCTCTCGG GCCTGCCCAC CCTGTTCTCC AGCTTGTAAA 601 GACGCCCACT GCTGGGGGGC CAGCTCCGGG GACTGTCAGA GCTTGACGCG GACTGTCTGT 661 GCGGGGGGCT GTGCCCGCTG CAAGGGCCCA CAACCCACCG ACTGCTGCCA CGAGCAGTGT 721 GCTGCTGGCT GCACGGGCCC CAAGCACTCT GACTGCCTGG CCTGCCTTCA CTTCAACCAC 781 AGTGGCATCT GTGAGCTGCA CTGCCCAGCC CTGGTCACCT ACAACACGGA CACCTTCGAA 841 TCCATGCCCA ACCCTGAGGG CCGATATACC TTCGGGGCCA GCTGTGTGAC CTCCTGTCCC 901 TACAACTACC TGTCTACGGA TGTGGGATCC TGCACCCTGG TCTGTCCCCT GAACAACCAA 961 GAGGTGACGG CTGAGGATGG GACACAGCGG TGCGAGAAAT GCAGCAAGCC CTGTGCCCGA 1021 GTGTGCTACG GTCTGGGCAT GGAGCACCTG CGGGAGGTGA GAGCGGTCAC CAGTGCGAAC 1081 ATCCAGGAGT TTGCCGGCTG CAAGAAGATC TTTGGAAGCC TGGCATTTTT GCCAGAGAGC 1141 TTTGAGGGGG ACCCAGCCTC CAACACTGCC CCCCTACAGC CTGAGCAGCT CAGAGTGTTT 1201 GAGGCTCTGG AGGAGATCAC AGGTTACCTG TACATCTCAG CGTGGCCAGA CAGCCTGCCT 1261 AACCTCAGTG TCTTCCAGAA CCTGCGAGTA ATCCGGGGAC GAGTTCTGCA TGATGGTGCC 1321 TACTCGCTGA CCCTGCAAGG GCTGGGCATC AGCTGGCTGG GGCTGCGCTC GCTGCGGGAA 1381 CTGGGCAGTG GGCTGGCCCT CATCCACCGC AACGCCCGCC TTTGCTTCGT GCACACGGTG 1441 CCCTGGGACC AGCTCTTCCG GAACCCCCAC CAGGCCCTGC TCCATAGTGC CAACCGGCCA 1501 GAGGAGGAGT GCGTGGGCGA GGGCCTGGCC TGCTACCCGC TGTGCGCCCA TGGGCACTGC 1561 TGGGGTCCAG GGCCCACCCA GTGCGTCAAC TGCAGCCAAT TCCTTCGGGG CCAGGAGTGC 1621 GTGGAAGAAT GCCGAGTACT GCAGGGGCTG CCCCGAGAGT ATGTGAAGGA CAGGTACTGT 1681 CTGCCGTGCC ACTCAGAGTG TCAGCCCCAG AATGGCTCAG TGACCTGCTT CGGATCGGAG 1741 GCTGACCAGT GTGTGGCCTG CGCCCACTAC AAGGACCCTC CCTTCTGTGT GGCTCGCTGC 1801 CCCAGTGGTG TGAAACCTGA CCTGTCCTTC ATGCCCATCT GGAAGTTCGC AGATGAGGAG 1861 GGCACTTGCC AGCCGTGCCC CATCAACTGC ACCCACTCCT GTGCGGACCT GGACGAGAAG 1921 GGCTGTCCCG CCGAGCAGAG AGCCAGCCCT GTGACATCCA TCATTGCCGC TGTGGTGGGC 1981 ATTCTGCTGG CTGTGGTCGT GGGGCTGGTC CTCGGCATCC TGATCAAGCG AAGGCGGCAG 2041 AAGATCCGGA AGTACACTAT GCGGAGGCTG CTGCAGGAAA CCGAGCTGGT GGAGCCGCTG 2101 ACGCCTAGTG GAGCGATGCC CAACCAGGCT CAGATGCGGA TCCTGAAAGA GACAGAGCTG 2161 AGGAAGGTGA AGGTGCTTGG ATCCGGAGCT TTTGGCACAG TCTACAAGGG CATCTGGATC 2221 CCTGATGGGG AAAATGTGAA AATCCCAGTG GCCATCAAAG TGTCGAGGGA AAACACATCT 2281 CCCAAAGCCA ACAAAGAAAT CTTGGACGAA GCATATGTGA TGGCTGGAGT GGGCTCCCCG 2341 TATGTGTCCC GCCTCCTGGG CATCTGCCTG ACATCCACGG TGCAGCTGGT GACACAGCTT 2401 ATGCCCTACG GCTGCCTCTT AGACCATGTC CGAGAACACC GTGGGCGCCT GGGCTCCCAG 2461 GACTTGCTGA ACTGGTGTGT GCAGATTGCC AAGGGGATGA GCTACTTGGA GGATGTCCGG 2521 CTGGTGCACA GGGACCTGGC TGCCCGGAAT GTGCTGGTCA AGAGTCCCAA CCATGTCAAG 2581 ATTACAGATT TCGGGCTGGC TCGGTTGCTG GACATCGACG AGACAGAGTA CCATGCGGAT 2641 GGGGGCAAGG TGCCCATCAA GTGGATGGCG CTGGAGTCCA TTCTCCGCCG GCGGTTCACC 2701 CACCAGAGTG ATGTGTGGAG CTATGGTGTG ACTGTGTGGG AACTGATGAC TTTTGGGGCC 2761 AAACCTTATG ATGGGATCCC AGCCCGGGAG ATCCCTGACC TGCTGGAGAA GGGGGAACGG 2821 CTGCCCCAGC CCCCCATCTG CACCATTGAT GTCTACATGA TCATGGTCAA GTGCTGGATG 2881 ATAGACTCTG AATGCCGACC CCGGTTCCGG GAGTTGGTGG CCGAATTCTC ACGTATGGCC 2941 AGGGACCCCC AGCGCTTTGT GGTCATTCAG AATGAAGACT TGGGCCCCGC CAGCCCCTTG 3001 GACAGCACCT TCTACCGTTC ACTACTGGAA GATGATGACA TGGGGGACCT GGTGGATGCT 3061 GAGGAGTACC TGGTACCCCA GCAGGGTTTC TTCTGCCCAG AACCTACCCC AGGGGCTGGG 3121 GGCACTGCCC ACCGACGGCA CCGCAGCTCA TCCACCAGGA GTGGCGGTGG TGAGCTGACT 3181 CTAGGACTGG AGCCCTCCGA GGAGGAGCCC CCCAAGTCTC CACTGGCACC CTCAGAGGGC 3241 GCTGGCTCTG ACGTGTTTGA TGGTGACTTG GGAATGGGGG CAGCCAAGGG GCTGCAGAGC 3301 CTTCCCTCAC AGGACCCCAG CCCTCTCCAG CGGTACAGTG AGGACCCTAC GGTACCCTTG 3361 CCCCCTGAGA CTGATGGCTA CGTCGCCCCC CTGACCTGCA GCCCCCAGCC TGAATATGTG 3421 AACCAGCCAG AAGTTTGGCC GCAGCCCCCC TCGCCCCTAG AAGGCCCTCT GCCTCCTTCC 3481 CGACCGGCTG GTGCCACTCT GGAAAGGCCC AAGACTCTGT CCCCCAAGAC TCTCTCCCCT 3541 GGCAAGAATG GGGTTGTCAA AGACGTTTTT GCCTTTGGGA GTGCTGTGGA GAATCCGGAG 3601 TACCTGGCAC CCCGGGGCAG AGCTGCCCCT CAGCCCCACC CTCCTCCAGC CTTCAGCCCA 3661 GCCTTTGACA ACCTGTATTA CTGGGACCAG GATCCATCAG AGCGGGGCTC TCCACCCAGC 3721 ACCTTTGAAG GGACCCCTAC AGCAGAGAAC CCGGAGTACC TGGGGCTGGA CGTGCCAGTG 3781 TGA
Quality Control:cDNA sequencing results is available upon request.
