Human EPOR transcript variant 2 Gene cDNA Clone / ORF Clone

EPOR cDNA Clone Datasheet

EPOR cDNA Clone Product Information

• Gene Bank Ref.ID: NR_033663.1
• cDNA Size: 1008
• Gene Synonym: EPO-R; MGC138358; EPOR
• Source: Human

EPOR cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens erythropoietin receptor, transcript variant 2 DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

EPOR cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

EPOR cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

EPOR cDNA Clone Sequence and Quality Control

Sequence:

1        ATGACGTCTC ACATCCGCTA CGAGGTGGAC GTCTCGGCCG GCAACGGCGC AGGGAGCGTA
61       CAGAGGGTGG AGATCCTGGA GGGCCGCACC GAGTGTGTGC TGAGCAACCT GCGGGGCCGG
121      ACGCGCTACA CCTTCGCCGT CCGCGCGCGT ATGGCTGAGC CGAGCTTCGG CGGCTTCTGG
181      AGCGCCTGGT CGGAGCCTGT GTCGCTGCTG ACGCCTAGCG ACCTGGACCC CCTCATCCTG
241      ACGCTCTCCC TCATCCTCGT GGTCATCCTG GTGCTGCTGA CCGTGCTCGC GCTGCTCTCC
301      CACCGCCGGG CTCTGAAGCA GAAGATCTGG CCTGGCATCC CGAGCCCAGA GAGCGAGTTT
361      GAAGGCCTCT TCACCACCCA CAAGGGTAAC TTCCAGCTGT GGCTGTACCA GAATGATGGC
421      TGCCTGTGGT GGAGCCCCTG CACCCCCTTC ACGGAGGACC CACCTGCTTC CCTGGAAGTC
481      CTCTCAGAGC GCTGCTGGGG GACGATGCAG GCAGTGGAGC CGGGGACAGA TGATGAGGGC
541      CCCCTGCTGG AGCCAGTGGG CAGTGAGCAT GCCCAGGATA CCTATCTGGT GCTGGACAAA
601      TGGTTGCTGC CCCGGAACCC GCCCAGTGAG GACCTCCCAG GGCCTGGTGG CAGTGTGGAC
661      ATAGTGGCCA TGGATGAAGG CTCAGAAGCA TCCTCCTGCT CATCTGCTTT GGCCTCGAAG
721      CCCAGCCCAG AGGGAGCCTC TGCTGCCAGC TTTGAGTACA CTATCCTGGA CCCCAGCTCC
781      CAGCTCTTGC GTCCATGGAC ACTGTGCCCT GAGCTGCCCC CTACCCCACC CCACCTAAAG
841      TACCTGTACC TTGTGGTATC TGACTCTGGC ATCTCAACTG ACTACAGCTC AGGGGACTCC
901      CAGGGAGCCC AAGGGGGCTT ATCCGATGGC CCCTACTCCA ACCCTTATGA GAACAGCCTT
961      ATCCCAGCCG CTGAGCCTCT GCCCCCCAGC TATGTGGCTT GCTCTTAG

 

Quality Control:cDNA sequencing results is available upon request.