Mouse EPOR Gene cDNA Clone / ORF Clone

EPOR cDNA Clone Datasheet

EPOR cDNA Clone Product Information

• Gene Bank Ref.ID: NM_010149.3
• cDNA Size: 1524
• Gene Synonym: EPOR
• Source: Mouse

EPOR cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus erythropoietin receptor DNA.
• Vector: Cloning Vector: pMD18-T Simple Vector.

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

EPOR cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

EPOR cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

EPOR cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGACAAAC TCAGGGTGCC CCTCTGGCCT CGGGTAGGCC CCCTCTGTCT CCTACTTGCT
61       GGGGCAGCCT GGGCACCTTC ACCCAGCCTC CCGGACCCCA AGTTTGAGAG CAAAGCGGCC
121      CTGCTGGCAT CCCGGGGCTC CGAAGAACTT CTGTGCTTCA CCCAACGCTT GGAAGACTTG
181      GTGTGTTTCT GGGAGGAAGC GGCGAGCTCC GGGATGGACT TCAACTACAG CTTCTCATAC
241      CAGCTCGAGG GTGAGTCACG AAAGTCATGT AGCCTGCACC AGGCTCCCAC CGTCCGCGGC
301      TCCGTGCGTT TCTGGTGTTC ACTGCCAACA GCGGACACAT CGAGTTTTGT GCCGCTGGAG
361      CTGCAGGTGA CGGAGGCGTC CGGTTCTCCT CGCTATCACC GCATCATCCA TATCAATGAA
421      GTAGTGCTCC TGGACGCCCC CGCGGGGCTG CTGGCGCGCC GGGCAGAAGA GGGCAGCCAC
481      GTGGTGCTGC GCTGGCTGCC ACCTCCTGGA GCACCTATGA CCACCCACAT CCGATATGAA
541      GTGGACGTGT CGGCAGGCAA CCGGGCAGGA GGGACACAAA GGGTGGAGGT CCTGGAAGGC
601      CGCACTGAGT GTGTTCTGAG CAACCTGCGG GGCGGGACGC GCTACACCTT CGCTGTTCGA
661      GCGCGCATGG CCGAGCCGAG CTTCAGCGGA TTCTGGAGTG CCTGGTCTGA GCCCGCGTCA
721      CTACTGACCG CTAGCGACCT GGACCCTCTC ATCTTGACGC TGTCTCTCAT TCTGGTCCTC
781      ATCTCGCTGT TGCTGACGGT TCTGGCCCTG CTGTCCCACC GCCGGACTCT GCAGCAGAAG
841      ATCTGGCCTG GCATCCCAAG CCCAGAGAGC GAGTTTGAGG GTCTCTTCAC CACCCACAAG
901      GGTAACTTCC AGCTGTGGCT GCTGCAGCGT GATGGTTGTC TGTGGTGGAG CCCGGGCAGC
961      TCCTTCCCTG AGGATCCACC TGCCCACCTA GAGGTCCTCT CAGAGCCACG CTGGGCAGTG
1021     ACTCAGGCTG GGGACCCAGG GGCAGATGAT GAGGGGCCCT TACTGGAGCC GGTGGGCAGT
1081     GAGCATGCCC AGGACACCTA CTTGGTATTG GATAAGTGGT TGCTGCCCCG GACCCCATGC
1141     AGTGAGAACC TCTCAGGGCC TGGGGGCAGT GTGGACCCTG TGACTATGGA TGAAGCTTCA
1201     GAAACATCTT CCTGCCCGTC TGACTTGGCC TCAAAGCCCA GGCCAGAGGG CACCTCACCT
1261     TCCAGCTTTG AGTACACCAT CCTGGACCCC AGCTCTCAGC TCCTGTGCCC TCGGGCACTG
1321     CCTCCCGAGC TACCTCCCAC TCCACCTCAC TTGAAGTACC TATACCTTGT GGTGTCCGAT
1381     TCTGGCATCT CAACAGATTA CAGTTCGGGG GGCTCTCAGG GAGTCCACGG GGACTCATCT
1441     GATGGCCCCT ACTCCCACCC CTATGAGAAC AGCCTTGTCC CAGACTCAGA GCCTCTGCAT
1501     CCCGGCTATG TGGCCTGCTC CTAG

 

Quality Control:cDNA sequencing results is available upon request.