Mouse EPO Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
MG51099-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

EPO cDNA Clone Datasheet

EPO cDNA Clone Product Information

Gene Bank Ref.ID:	NM_007942.2
cDNA Size:	579
Gene Synonym:	Epo
Source:	Mouse

EPO cDNA Clone Description

cDNA Description:	ORF Clone of Mus musculus erythropoietin DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

EPO cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

EPO cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

EPO cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGGGTGC CCGAACGTCC CACCCTGCTG CTTTTACTCT CCTTGCTACT GATTCCTCTG
61       GGCCTCCCAG TCCTCTGTGC TCCCCCACGC CTCATCTGCG ACAGTCGAGT TCTGGAGAGG
121      TACATCTTAG AGGCCAAGGA GGCAGAAAAT GTCACGATGG GTTGTGCAGA AGGTCCCAGA
181      CTGAGTGAAA ATATTACAGT CCCAGATACC AAAGTCAACT TCTATGCTTG GAAAAGAATG
241      GAGGTGGAAG AACAGGCCAT AGAAGTTTGG CAAGGCCTGT CCCTGCTCTC AGAAGCCATC
301      CTGCAGGCCC AGGCCCTGCT AGCCAATTCC TCCCAGCCAC CAGAGACCCT TCAGCTTCAT
361      ATAGACAAAG CCATCAGTGG TCTACGTAGC CTCACTTCAC TGCTTCGGGT ACTGGGAGCT
421      CAGAAGGAAT TGATGTCGCC TCCAGATACC ACCCCACCTG CTCCACTCCG AACACTCACA
481      GTGGATACTT TCTGCAAGCT CTTCCGGGTC TACGCCAACT TCCTCCGGGG GAAACTGAAG
541      CTGTACACGG GAGAGGTCTG CAGGAGAGGG GACAGGTGA

Quality Control:cDNA sequencing results is available upon request.