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> Gene Products > Other Species > Cynomolgus monkey EPO Gene cDNA Clone / ORF Clone Cynomolgus monkey EPO Gene cDNA Clone / ORF Clone
| Catalog | Vector (Price) | Quantity | In Stock | Operation |
| CG90133-G |
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* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. |
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EPO cDNA Clone Datasheet
EPO cDNA Clone Product Information
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cDNA Size: |
579 |
| Gene Synonym: |
EPO |
| Source: | Cynomolgus monkey |
EPO cDNA Clone Description
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cDNA Description: |
ORF Clone of Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) erythropoietin DNA. |
| Vector: | Cloning Vector: pGEM-T Vector
Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG |
| Sequence Description: | Identical with the Gene Bank Ref. ID sequence except for the point mutations: 97 G/A, 49 G/C, 243 A/G resulting in the amino acid Val substitution by Ile, Val substitution by Leu, Ile substitution by Met and 48 C/G not causing the amino acid variation. Please check the sequence information before order. |
EPO cDNA Clone Shipping
- 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
- It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.
EPO cDNA Clone Usage Protocol
- Cut along the circle edge of the spot (arrow points to) to get the filter paper.
- Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O Ensure the filter is completely immersed in the H2O by briefly centrifuging the tube for 10 seconds.
- Transfer the tube to incubation at 95℃ for 15-30 min.
- At the end of the incubation pulse vortex, or gently tap the tube.
- Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.
EPO cDNA Clone Sequence and Quality Control
Sequence:
1 ATGGGGGTGC ACGAATGTCC TGCCTGGCTG TGGCTTCTCC TGTCTCTGCT GTCGCTCCCT 61 CTGGGCCTCC CAGTCCCGGG CGCCCCACCA CGCCTCATCT GTGACAGCCG AGTCCTGGAG 121 AGGTACCTCT TGGAGGCCAA GGAGGCCGAG AATGTCACGA TGGGCTGTTC CGAAAGCTGC 181 AGCTTGAATG AGAATATCAC CGTCCCAGAC ACCAAAGTTA ACTTCTATGC CTGGAAGAGG 241 ATGGAGGTCG GGCAGCAGGC TGTAGAAGTC TGGCAGGGCC TGGCCCTGCT CTCAGAAGCT 301 GTCCTGCGGG GCCAGGCCGT GTTGGCCAAC TCTTCCCAGC CTTTCGAGCC CCTGCAGCTG 361 CACATGGATA AAGCCATCAG TGGCCTTCGC AGCATCACCA CTCTGCTTCG GGCGCTGGGA 421 GCCCAGGAAG CCATCTCCCT CCCAGATGCG GCCTCGGCTG CTCCACTCCG AACCATCACT 481 GCTGACACTT TCTGCAAACT CTTCCGAGTC TACTCCAATT TCCTCCGGGG AAAGCTGAAG 541 CTGTACACGG GGGAGGCCTG CAGGAGAGGG GACAGATGA
Quality Control:cDNA sequencing results is available upon request.
