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Cynomolgus monkey EPO Gene cDNA Clone (full-length ORF Clone)
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Cynomolgus monkey EPO Gene cDNA Clone (full-length ORF Clone)

Catalog Vector (Price) Quantity In Stock Operation
CG90133-G





  YES

*  pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors.
    Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

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EPO cDNA Clone Datasheet

 

EPO cDNA Clone Product Information

Gene Bank Ref.ID:

unsubmitted

cDNA Size:

579

Gene Synonym:

EPO

Source: Cynomolgus monkey

EPO cDNA Clone Description

cDNA Description:

ORF Clone of Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) erythropoietin DNA.

Vector: Cloning Vector: pGEM-T Vector

Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

Sequence Description: Identical with XM_001102109.2 [ Macaca mulatta (Rhesus monkey) ]:97 G/A, 49 G/C, 243 A/G resulting in the amino acid Val substitution by Ile, Val substitution by Leu, Ile substitution by Met and 48 C/G not causing the amino acid variation.
Please check the sequence information before order.

EPO cDNA Clone Shipping

  1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
  2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

EPO cDNA Clone Usage Protocol

  1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
  2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O Ensure the filter is completely immersed in the H2O Briefly centrifuge the tube for 10 seconds.
  3. Incubate the tube at 95℃ for 15-30 min.
  4. At the end of the incubation pulse vortex, or gently tap the tube.
  5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

EPO cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGGGTGC ACGAATGTCC TGCCTGGCTG TGGCTTCTCC TGTCTCTGCT GTCGCTCCCT
61       CTGGGCCTCC CAGTCCCGGG CGCCCCACCA CGCCTCATCT GTGACAGCCG AGTCCTGGAG
121      AGGTACCTCT TGGAGGCCAA GGAGGCCGAG AATGTCACGA TGGGCTGTTC CGAAAGCTGC
181      AGCTTGAATG AGAATATCAC CGTCCCAGAC ACCAAAGTTA ACTTCTATGC CTGGAAGAGG
241      ATGGAGGTCG GGCAGCAGGC TGTAGAAGTC TGGCAGGGCC TGGCCCTGCT CTCAGAAGCT
301      GTCCTGCGGG GCCAGGCCGT GTTGGCCAAC TCTTCCCAGC CTTTCGAGCC CCTGCAGCTG
361      CACATGGATA AAGCCATCAG TGGCCTTCGC AGCATCACCA CTCTGCTTCG GGCGCTGGGA
421      GCCCAGGAAG CCATCTCCCT CCCAGATGCG GCCTCGGCTG CTCCACTCCG AACCATCACT
481      GCTGACACTT TCTGCAAACT CTTCCGAGTC TACTCCAATT TCCTCCGGGG AAAGCTGAAG
541      CTGTACACGG GGGAGGCCTG CAGGAGAGGG GACAGATGA

 

Quality Control:cDNA sequencing results is available upon request.

 

EPO / Erythropoietin related areas, pathways, and other information

 
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"