Mouse EPO / Erythropoietin Protein (His Tag)

Erythropoietin Protein Datasheet

EPO / Erythropoietin Protein Product Information

• Synonym: EPO
• Protein Construction: A DNA sequence encoding the mouse EPO (P07321) (Met1- Arg192) was expressed with a C-terminal polyhistidine tag.
• Source: Mouse
• Expression Host: Human Cells

EPO / Erythropoietin Protein QC Testing

• Purity: >96% as determined by SDS-PAGE
• Bio-activity: 1. Measured by its ability to bind biotinylated human EPOR-His (Cat:10707-H08H) in a functional ELISA.
  2. Measured by its ability to bind mouse EPOR-Fch (Cat:50031-M03H) in a functional ELISA.
  3. Measured by its ability to bind human EPOR & CD131-Fc (Cat:CT010-H02H) in a functional ELISA.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Ala 27
• Molecular Mass: The recombinant mouse EPO comprises 177 amino acids and has a predicted molecular mass of 20 kDa. The apparent molecular mass of the protein is approximately 37 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: EPO / Erythropoietin protein

EPO / Erythropoietin Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

EPO / Erythropoietin Protein Description

Erythropoietin, also known as EPO, is a secreted protein which belongs to the EPO / TPO family. Erythropoietin / EPO is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Erythropoietin / EPO is produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals. EPO is produced mainly by peritubular fibroblasts of the renal cortex. It is synthesized by renal peritubular cells in adults, with a small amount being produced in the liver. Erythropoietin / EPO is a glycoprotein hormone that controls erythropoiesis, or red blood cell production. It is a cytokine for erythrocyte (red blood cell) precursors in the bone marrow. Erythropoietin / EPO is the hormone that regulates red blood cell production. It plays an important role in the brain's response to neuronal injury. Erythropoietin / EPO is also involved in the wound healing process. Regulation is believed to rely on a feed-back mechanism measuring blood oxygenation. Erythropoietin / EPO binds to the erythropoietin receptor (EPOR) on the red cell surface and activates a JAK2 cascade. EPOR is also found in a large number of tissues such as bone marrow cells and peripheral/central nerve cells, many of which activate intracellular biological pathways upon binding with EPO. Genetic variation in EPO is associated with susceptbility to microvascular complications of diabetes type 2 (MVCD2). These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus.

References

1. Lin FK. et al., 1985, Proc Natl Acad Sci. 82 (22): 7580-4.
2. Fisher JW. et al.,1996, British journal of haematology. 95 (1): 27-32.
3. Macdougall IC. et al., 2000, Semin Nephrol. 20 (4): 375-81.
4. Siren AL. et al., 2001. Proc Natl Acad Sci. 98 (7): 4044-9.
5. Haroon ZA. et al.,2003, Am J Pathol. 163 (3): 993-1000.
6. Jelkmann W. et al., 2007, Eur J Haematol.78 (3): 183-205.