EPHB3 / HEK2 (aa 585-998) Protein (GST Tag)

EPH receptor B3 Protein Datasheet

EPHB3 / HEK2 Protein Product Information

• Synonym: EPHB3, ETK2, HEK2, TYRO6
• Protein Construction: A DNA sequence encoding the human EPHB3 (P54753) (Gln585-Val998) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
• Source: Human
• Expression Host: Baculovirus-Insect cells

EPHB3 / HEK2 Protein QC Testing

• Purity: > 90% as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant human EPHB3 /GST chimera consists of 651 amino acids and has a calculated molecular mass of 74.7 kDa. The recombinant protein migrates as an approximately 64 kDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH7.4, 10%gly.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: EPHB3 / HEK2 protein

EPHB3 / HEK2 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

EPHB3 / HEK2 Protein Description

Ephrin type-B receptor 3, EPH-like kinase 2, Tyrosine-protein kinase TYRO6, EPHB3, ETK2, HEK2 and TYRO6, is a single-pass type I  membrane protein which belongs to the protein kinase superfamily, tyr protein kinase family, ephrin receptor subfamily. EPHB3 contains two fibronectin type-III domains, one protein kinase domain and one SAM (sterile alpha motif) domain. EphB3 is initially expressed in the sclerotome, but later is expressed predominantly in the dermatome. It is prominent expression is also detected in the developing heart, liver, posterior ventral limb bud mesenchyme, pharyngeal arches, and head mesenchyme. The EphB3 receptors are expressed during embryonic development in multiple regions of the central nervous system. The spatial profile of EphB3 receptors is co-localized to regions of the brain that had a high level of EphB3 receptor binding ligands. Its expression pattern suggests that EphB3 may play a role in the maintenance of mature neuronal connections or re-arrangement of synaptic connections during late stages of development. EphB3-ephrin-B interaction promotes MET by re-establishing epithelial cell-cell junctions and such an MET-promoting effect contributes to EphB3-mediated tumor suppression.

References

1. Baker RK. et al., 2001, Mech Dev. 104 (1-2): 129-32.
2. Willson CA. et al., 2003, Cell Transplant. 12 (3): 279-90.
3. Willson CA. et al., 2006, J Mol Histol. 37 (8-9): 369-80.
4. Risley M. et al., 2009, Mech Dev. 126 (3-4): 230-9.
5. Chiu ST. et al., 2009, Carcinogenesis. 30 (9): 1475-86.