EphB1 Protein, Human, Recombinant (aa 565-984, His & GST Tag)

Cat: 11963-H20B1
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EphB1 Protein, Human, Recombinant (aa 565-984, His & GST Tag) Product Information
Purity
> 95 % as determined by SDS-PAGE
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Activity
The specific activity was determined to be 140 nmol/min/mg using Poly(Glu:Tyr) 4:1 as substrate.
Protein Construction
A DNA sequence encoding the human EPHB1 (AAI11745.1) (Arg565-Ala984) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Accession#
Expressed Host
Baculovirus-Insect Cells
Species
Human
Predicted N Terminal
Met
Molecule Mass
The recombinant human EPHB1 /GST chimera consists of 657 amino acids and has a calculated molecular mass of 75.3 kDa. The recombinant protein migrates as an approximately 66 kDa band in SDS-PAGE under reducing conditions.
Formulation
Supplied as sterile 20mM Tris, 500mM NaCl, 3mM DTT, pH 8.0, 10% gly
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.
Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -70℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
EphB1 Protein, Human, Recombinant (aa 565-984, His & GST Tag) in SDS-PAGE
Human EphB1/Eph Receptor B1 Protein 9906
EphB1 Protein, Human, Recombinant (aa 565-984, His & GST Tag) Alternative Names
ELK Protein, Human;EPHT2 Protein, Human;Hek6 Protein, Human;NET Protein, Human
EphB1 Background Information

Ephrin type-B receptor 1, also known as EphB1, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA10, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. EphB2 receptor tyrosine kinase phosphorylates syndecan-2 and that this phosphorylation event is crucial for syndecan-2 clustering and spine formation. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs). Ligand-mediated activation of Ephs induce various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer. EphB receptor tyrosine kinases are enriched at synapses, suggesting that these receptors play a role in synapse formation or function. We find that EphrinB binding to EphB induces a direct interaction of EphB with NMDA-type glutamate receptors. This interaction occurs at the cell surface and is mediated by the extracellular regions of the two receptors, but does not require the kinase activity of EphB.

Full Name
EPH receptor B1
References
  • Dalva MB, et al. (2000) EphB receptors interact with NMDA receptors and regulate excitatory synapse formation. Cell. 103(6): 945-56.
  • Takasu MA, et al. (2002) Modulation of NMDA receptor-dependent calcium influx and gene expression through EphB receptors. Science. 295(5554): 491-5.
  • Adams RH, et al. (1999) Roles of ephrinB ligands and EphB receptors in cardiovascular development: demarcation of arterial/venous domains, vascular morphogenesis, and sprouting angiogenesis. Genes Dev. 13(3): 295-306.
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