Rat EPHA4 Protein (His Tag)

Eph receptor A4 Protein Datasheet

EPHA4 Protein Product Information

• Synonym: EPHA4
• Protein Construction: A DNA sequence encoding the rat EPHA4(D3ZZK3)(Met1-Thr547) was expressed, fused with a polyhistidine tag at the C-terminus.
• Source: Rat
• Expression Host: Human Cells

EPHA4 Protein QC Testing

• Purity: > 97 % as determined by SDS-PAGE
• Bio-activity: Measured by its binding ability in a functional ELISA.
  Immobilized recombinant Rat EPHA4 Protein (His Tag) (Cat: 80123-R08H) at 10 µg/mL (100 μl/well) can bind Recombinant rat EFNA5-Fc (Cat: 80105-R02H) with a linear range of 7.8-12.5 ng/ml.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Val 20
• Molecular Mass: The recombinant rat EPHA4 comprises 539 amino acids and predicts a molecular mass of 59.8 kDa. The apparent molecular mass of the recombinant protein is approximately 71 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: EPHA4 protein

EPHA4 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

EPHA4 Protein Description

Ephrin type-A receptor 4, also known as Tyrosine-protein kinase receptor SEK, Tyrosine-protein kinase TYRO1, EPH-like kinase 8, and EPHA4, is a single-pass type I membrane protein which belongs to the protein kinase superfamily and ephrin receptor subfamily. EPHA4 contains two fibronectin type-III domains, one protein kinase domain and one SAM (sterile alpha motif) domain. EPHA4 is a receptor for members of the ephrin-A family. EPHA4 binds to ephrin-A1, ephrin-A4 and ephrin-A5. It binds more poorly to ephrin-A2 and ephrin-A3. EPHA4 may play a role in a signal transduction process involved in hindbrain pattern formation.

The ephrins (Eph) and Eph receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosyl- phosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. All ephrins share a conserved extracellular sequence, which most likely corresponds to the receptor-binding domain. In molecular biology, ephrins and Eph receptors are components of cell signaling pathways involved in animal development, and implicated in some cancers. Eph receptors are classified as receptor tyrosine kinases (RTKs), and form the largest sub-family of RTKs.

References

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7. Heibeck TH. et al., 2009, J. Proteome Res. 8: 3852-61.