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Human EPHA3 Gene cDNA Clone / ORF Clone
| Catalog | Vector (Price) | Quantity | In Stock | Operation |
| HG11459-M |
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YES |  |
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* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. |
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EPHA3 cDNA Clone Datasheet
EPHA3 cDNA Clone Product Information
| Gene Bank Ref.ID: | NM_005233.5 |
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cDNA Size: |
2952 |
| Gene Synonym: |
ETK; HEK; ETK1; HEK4; TYRO4; EPHA3 |
| Source: | Human |
EPHA3 cDNA Clone Description
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cDNA Description: |
ORF Clone of Homo sapiens EPH receptor A3 DNA. |
| Vector: | Cloning Vector: pMD18-T Simple Vector.
Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG |
| Sequence Description: | Same as Gene Bank Ref. ID sequence with the nucleotide (2750-2846) deletion mutation and the point mutation 21C/A not causing the amino acid variation. |
EPHA3 cDNA Clone Shipping
- 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
- It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.
EPHA3 cDNA Clone Usage Protocol
- Cut along the circle edge of the spot (arrow points to) to get the filter paper.
- Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O . Ensure the filter paper is completely immersed in the H2O by briefly centrifuging the tube for 10 seconds.
- Transfer the tube to incubation at 95℃ for 15-30 min.
- At the end of the incubation pulse vortex, or gently tap the tube.
- Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.
EPHA3 cDNA Clone Sequence and Quality Control
Sequence:
1 ATGGATTGTC AGCTCTCCAT ACTCCTCCTT CTCAGCTGCT CTGTTCTCGA CAGCTTCGGG 61 GAACTGATTC CGCAGCCTTC CAATGAAGTC AATCTACTGG ATTCAAAAAC AATTCAAGGG 121 GAGCTGGGCT GGATCTCTTA TCCATCACAT GGGTGGGAAG AGATCAGTGG TGTGGATGAA 181 CATTACACAC CCATCAGGAC TTACCAGGTG TGCAATGTCA TGGACCACAG TCAAAACAAT 241 TGGCTGAGAA CAAACTGGGT CCCCAGGAAC TCAGCTCAGA AGATTTATGT GGAGCTCAAG 301 TTCACTCTAC GAGACTGCAA TAGCATTCCA TTGGTTTTAG GAACTTGCAA GGAGACATTC 361 AACCTGTACT ACATGGAGTC TGATGATGAT CATGGGGTGA AATTTCGAGA GCATCAGTTT 421 ACAAAGATTG ACACCATTGC AGCTGATGAA AGTTTCACTC AAATGGATCT TGGGGACCGT 481 ATTCTGAAGC TCAACACTGA GATTAGAGAA GTAGGTCCTG TCAACAAGAA GGGATTTTAT 541 TTGGCATTTC AAGATGTTGG TGCTTGTGTT GCCTTGGTGT CTGTGAGAGT ATACTTCAAA 601 AAGTGCCCAT TTACAGTGAA GAATCTGGCT ATGTTTCCAG ACACGGTACC CATGGACTCC 661 CAGTCCCTGG TGGAGGTTAG AGGGTCTTGT GTCAACAATT CTAAGGAGGA AGATCCTCCA 721 AGGATGTACT GCAGTACAGA AGGCGAATGG CTTGTACCCA TTGGCAAGTG TTCCTGCAAT 781 GCTGGCTATG AAGAAAGAGG TTTTATGTGC CAAGCTTGTC GACCAGGTTT CTACAAGGCA 841 TTGGATGGTA ATATGAAGTG TGCTAAGTGC CCGCCTCACA GTTCTACTCA GGAAGATGGT 901 TCAATGAACT GCAGGTGTGA GAATAATTAC TTCCGGGCAG ACAAAGACCC TCCATCCATG 961 GCTTGTACCC GACCTCCATC TTCACCAAGA AATGTTATCT CTAATATAAA CGAGACCTCA 1021 GTTATCCTGG ACTGGAGTTG GCCCCTGGAC ACAGGAGGCC GGAAAGATGT TACCTTCAAC 1081 ATCATATGTA AAAAATGTGG GTGGAATATA AAACAGTGTG AGCCATGCAG CCCAAATGTC 1141 CGCTTCCTCC CTCGACAGTT TGGACTCACC AACACCACGG TGACAGTGAC AGACCTTCTG 1201 GCACATACTA ACTACACCTT TGAGATTGAT GCCGTTAATG GGGTGTCAGA GCTGAGCTCC 1261 CCACCAAGAC AGTTTGCTGC GGTCAGCATC ACAACTAATC AGGCTGCTCC ATCACCTGTC 1321 CTGACGATTA AGAAAGATCG GACCTCCAGA AATAGCATCT CTTTGTCCTG GCAAGAACCT 1381 GAACATCCTA ATGGGATCAT ATTGGACTAC GAGGTCAAAT ACTATGAAAA GCAGGAACAA 1441 GAAACAAGTT ATACCATTCT GAGGGCAAGA GGCACAAATG TTACCATCAG TAGCCTCAAG 1501 CCTGACACTA TATACGTATT CCAAATCCGA GCCCGAACAG CCGCTGGATA TGGGACGAAC 1561 AGCCGCAAGT TTGAGTTTGA AACTAGTCCA GACTCTTTCT CCATCTCTGG TGAAAGTAGC 1621 CAAGTGGTCA TGATCGCCAT TTCAGCGGCA GTAGCAATTA TTCTCCTCAC TGTTGTCATC 1681 TATGTTTTGA TTGGGAGGTT CTGTGGCTAT AAGTCAAAAC ATGGGGCAGA TGAAAAAAGA 1741 CTTCATTTTG GCAATGGGCA TTTAAAACTT CCAGGTCTCA GGACTTATGT TGACCCACAT 1801 ACATATGAAG ACCCTACCCA AGCTGTTCAT GAGTTTGCCA AGGAATTGGA TGCCACCAAC 1861 ATATCCATTG ATAAAGTTGT TGGAGCAGGT GAATTTGGAG AGGTGTGCAG TGGTCGCTTA 1921 AAACTTCCTT CAAAAAAAGA GATTTCAGTG GCCATTAAGA CCCTGAAAGT TGGCTACACA 1981 GAAAAGCAGA GGAGAGACTT CCTGGGAGAA GCAAGCATTA TGGGACAGTT TGACCACCCC 2041 AATATCATTC GACTGGAAGG AGTTGTTACC AAAAGTAAGC CAGTTATGAT TGTCACAGAA 2101 TACATGGAGA ATGGTTCCTT GGATAGTTTC CTACGTAAAC ACGATGCCCA GTTTACTGTC 2161 ATTCAGCTAG TGGGGATGCT TCGAGGGATA GCATCTGGCA TGAAGTACCT GTCAGACATG 2221 GGCTATGTTC ACCGAGACCT CGCTGCTCGG AACATCTTGA TCAACAGTAA CTTGGTGTGT 2281 AAGGTTTCTG ATTTCGGACT TTCGCGTGTC CTGGAGGATG ACCCAGAAGC TGCTTATACA 2341 ACAAGAGGAG GGAAGATCCC AATCAGGTGG ACATCACCAG AAGCTATAGC CTACCGCAAG 2401 TTCACGTCAG CCAGCGATGT ATGGAGTTAT GGGATTGTTC TCTGGGAGGT GATGTCTTAT 2461 GGAGAGAGAC CATACTGGGA GATGTCCAAT CAGGATGTAA TTAAAGCTGT AGATGAGGGC 2521 TATCGACTGC CACCCCCCAT GGACTGCCCA GCTGCCTTGT ATCAGCTGAT GCTGGACTGC 2581 TGGCAGAAAG ACAGGAACAA CAGACCCAAG TTTGAGCAGA TTGTTAGTAT TCTGGACAAG 2641 CTTATCCGGA ATCCCGGCAG CCTGAAGATC ATCACCAGTG CAGCCGCAAG GCCATCAAAC 2701 CTTCTTCTGG ACCAAAGCAA TGTGGATATC ACTACCTTCC GCACAACAGG TGACTGGCTT 2761 AATGGTGTCT GGACAGCACA CTGCAAGGAA ATCTTCACGG GTGTGGAGTA CAGTTCTTGT 2821 GACACAATAG CCAAGATTTC CACAGATGAC ATGAAAAAGG TTGGTGTCAC CGTGGTTGGG 2881 CCACAGAAGA AGATCATCAG TAGCATTAAA GCTCTAGAAA CGCAATCAAA GAATGGCCCA 2941 GTTCCCGTGT AA
Quality Control:cDNA sequencing results is available upon request.
