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>Human EphA2 Protein (aa 585-976, His & GST Tag)
|Catalog||Size (Price)||Quantity||In Stock||Operation|
|Form: Liquid||Shipping fee: $300|
EPH receptor A2 Protein Datasheet
EPHA2 Protein Price Inquiry ( Available Sizes )
EPHA2 Protein Product Information
|Synonym :||EPHA2, ECK|
A DNA sequence encoding the human EPHA2 (P29317) (Leu585-Ile976) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
|Expression Host:||Baculovirus-Insect cells|
EPHA2 Protein QC Testing
|Purity:||> 95% as determined by SDS-PAGE||SDS-PAGE:
|The specific activity was determined to be 50 nmol/min/mg using Poly(Glu:Tyr) 4:1 as substrate (see protocol for kinase assay).|
|Endotoxin:||< 1.0 EU per μg of the protein as determined by the LAL method|
|Stability:||Samples are stable for up to twelve months from date of receipt at -70℃|
|Predicted N terminal:||Met|
The recombinant human EPHA2 /GST chimera consists of 629 amino acids and has a calculated molecular mass of 72.1 kDa. The recombinant protein migrates as an approximately 62 kDa band in SDS-PAGE under reducing conditions.
|Formulation:||Supplied as sterile 20mM Tris, 500mM NaCl, 3mMDTT, pH8.5, 10%gly.|
EPHA2 Protein Usage Guide
|Shipping:||It is shipped out with blue ice.|
|Storage:||Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.|
EPHA2 Protein Related Products & Topics
|Molecule||Species||Description //For Detailed Info. and Price------CLICK!||Cat. No|
|EphA2||Human||EPHA2 (aa 585-976) Protein, Recombinant, with GST Tag||13926-H20B1|
|EphA2||Mouse||EphA2 Protein, Recombinant||50586-M08H|
|Molecule||Application||Description //For Detailed Info. and Price------CLICK!||Cat. No|
|Mouse EphA2||WB, ELISA||EphA2 Antibody||50586-RP01|
|Mouse EphA2||WB, ELISA||EphA2 Antibody (Antigen Affinity Purified)||50586-RP02|
EPHA2 Protein Description
Ephrin type-A receptor 2, also known as Epithelial cell kinase, Tyrosine-protein kinase receptor ECK and EPHA2, is a single-pass type I membrane protein which belongs to the protein kinase superfamily, tyr protein kinase family and ephrin receptor subfamily. EPHA2 is a receptor for members of the ephrin-A family. EPHA2 binds to ephrin-A1, ephrin-A3, ephrin-A4 and ephrin-A5. It plays an important role in angiogenesis and tumor neovascularization. The recruitement of VAV2, VAV3 and PI3-kinase p85 subunit by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly. Genetic variations in EPHA2 are the cause of susceptibility to cataract cortical age-related type 2. The ephrins (Eph) and Eph receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosyl- phosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. All ephrins share a conserved extracellular sequence, which most likely corresponds to the receptor-binding domain. In molecular biology, ephrins and Eph receptors are components of cell signaling pathways involved in animal development, and implicated in some cancers. Eph receptors are classified as receptor tyrosine kinases (RTKs), and form the largest sub-family of RTKs.
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