EPHA2 (aa 585-976) Protein (GST Tag)

EPH receptor A2 Protein Datasheet

EPHA2 Protein Product Information

• Synonym: EPHA2, ECK
• Protein Construction: A DNA sequence encoding the human EPHA2 (P29317) (Leu585-Ile976) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
• Source: Human
• Expression Host: Baculovirus-Insect cells

EPHA2 Protein QC Testing

• Purity: > 95% as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant human EPHA2 /GST chimera consists of 629 amino acids and has a calculated molecular mass of 72.1 kDa. The recombinant protein migrates as an approximately 62 kDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile 20mM Tris, 500mM NaCl, 3mMDTT, pH8.5, 10%gly.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: EPHA2 protein

EPHA2 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

EPHA2 Protein Description

Ephrin type-A receptor 2, also known as Epithelial cell kinase, Tyrosine-protein kinase receptor ECK and EPHA2, is a single-pass type I  membrane protein which belongs to the protein kinase superfamily, tyr protein kinase family and ephrin receptor subfamily. EPHA2 is a receptor for members of the ephrin-A family. EPHA2 binds to ephrin-A1, ephrin-A3, ephrin-A4 and ephrin-A5. It plays an important role in angiogenesis and tumor neovascularization. The recruitement of VAV2, VAV3 and PI3-kinase p85 subunit by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly. Genetic variations in EPHA2 are the cause of susceptibility to cataract cortical age-related type 2. The ephrins (Eph) and Eph receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosyl- phosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. All ephrins share a conserved extracellular sequence, which most likely corresponds to the receptor-binding domain. In molecular biology, ephrins and Eph receptors are components of cell signaling pathways involved in animal development, and implicated in some cancers. Eph receptors are classified as receptor tyrosine kinases (RTKs), and form the largest sub-family of RTKs.

References

1. Nowakowski J. et al., 2002, Structure. 10: 1659-67.
2. Murai KK. et al., 2003, Journal of Cell Science. 116 (14): 2823-32.
3. The MGC Project Team. 2004, Genome Res. 14: 2121-7.
4. Rikova K. et al., 2007, Cell. 131: 1190-203.
5. Zhang G. et al., 2008, Cancer Res. 68: 1691-6.
6. Himanen JP. et al., 2009, EMBO Rep. 10: 722-8.
7. Heibeck TH. et al., 2009, J Proteome Res. 8: 3852-61.