Mouse EPHA1 / EPH receptor A1 Protein (His Tag)

Eph receptor A1 Protein Datasheet

EPHA1 / EPH receptor A1 Protein Product Information

• Synonym: 5730453L17Rik, AL033318, Eph, Esk
• Protein Construction: A DNA sequence encoding the extracellular domain of mouse EPHA1 (Q60750) (Met 1-Glu 548) was expressed, with a C-terminal polyhistidine tag.
• Source: Mouse
• Expression Host: Human Cells

EPHA1 / EPH receptor A1 Protein QC Testing

• Purity: > 97 % as determined by SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Arg 25
• Molecular Mass: The secreted recombinant mouse EPHA1 comprises 535 amino acids and has a calculated molecular mass of 58.6 kDa. As a result of glycosylation, the apparent molecular mass of rmEPHA1 is approximately 68 kDa in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: EPHA1 / EPH receptor A1 protein

EPHA1 / EPH receptor A1 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

EPHA1 / EPH receptor A1 Protein Description

EPHA1 or EPH receptor A1 belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. Eph receptor tyrosine kinases and their membrane-bound ligands, ephrins, have key roles in patterning and morphogenesis. An important role of Eph receptors and ephrins is to mediate cell-contact-dependent repulsion. Complementary and overlapping gradients of expression underlie establishment of a topographic map of neuronal projections in the retinotectal system. Eph receptors and ephrins also act at boundaries to channel neuronal growth cones along specific pathways, restrict the migration of neural crest cells, and via bidirectional signaling prevent intermingling between hindbrain segments. Intriguingly, Eph receptors and ephrins can also trigger an adhesive response of endothelial cells and are required for the remodeling of blood vessels.Eph receptors and ephrins have emerged as key regulators of the repulsion and adhesion of cells that underlie the establishment, maintainence, and remodeling of patterns of cellular organization. The ephrins and Eph receptors are implicated as positional labels that may guide the development of neural topographic maps.

References

1. Flanagan JG. et al., 1998,  Annual Review of Neuroscience. 21: 309-45.
2. Wilkinson DG. 2000,  International Review of Cytology. 196: 177-244.
3. Zhou R. 1998, Pharmacol. 77 (3): 151–81.