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The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.
|Rat EPCAM Gene cDNA Clone (full-length ORF Clone), expression ready, FLAG-tagged||RG80306-G-F|
|Rat EPCAM Gene cDNA Clone (full-length ORF Clone), expression ready, His-tagged||RG80306-G-H|
|Rat EPCAM Gene cDNA Clone (full-length ORF Clone), expression ready, Myc-tagged||RG80306-G-M|
|Rat EPCAM Gene cDNA Clone (full-length ORF Clone), expression ready, untagged||RG80306-G-N|
|Rat EPCAM Gene cDNA Clone (full-length ORF Clone), expression ready, HA-tagged||RG80306-G-Y|
Epithelial Cell Adhesion Molecule (EpCAM), also known as GA733-2 antigen, is a type â… transmembrane glycoprotein composed of an extracellular domain with two EGF-Like repeats and a cystenin-rich region, a transmembrane domain and a cytoplasmic domain. It modulates cell adhesion and proliferation. Its overexpression has been detected in many epithelial tumours and has been associated with high stage, high grade and a worse survival in some tumour types. EpCAM has been shown to function as a calcium-independent homophilic cell adhesion molecule that does not exhibit any obvious relationship to the four known cell adhesion molecule superfamilies. However, recent insights have revealed that EpCAM participates in not only cell adhesion, but also in proliferation, migration and differentiation of cells. In addition, recent study revealed that EpCAM is the Wnt-beta-catenin signaling target gene and may be used to facilitate prognosis. It has oncogenic potential and is activated by release of its intracellular domain, which can signal into the cell nucleus by engagement of elements of the wnt pathway.