ENTPD3 / NTPDase3 / CD39L3 (aa 44-485) Protein (His Tag)

Ectonucleoside triphosphate diphosphohydrolase 3 Protein Datasheet

ENTPD3 / NTPDase3 / CD39L3 Protein Product Information

• Synonym: CD39L3, HB6, NTPDase-3
• Protein Construction: A DNA sequence encoding the human ENTPD3 (O75355) extracellular domain （Gln 44 -Pro 485）was fused with a polyhistidine tag at the C-terminus and a signal peptide at the N-terminus.
• Source: Human
• Expression Host: Baculovirus-Insect cells

ENTPD3 / NTPDase3 / CD39L3 Protein QC Testing

• Purity: > 97 % as determined by SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Gln 44
• Molecular Mass: The recombinant human ENTPD3 (aa44-485) consists of 452 amino acids and predicts a molecular mass of 51 kDa. It migrates as an approximately 50 KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from 0.2μm filtered solution of 20mM Tris, 500mM NaCl, pH 7.4, 10% gly
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: ENTPD3 / NTPDase3 / CD39L3 protein

ENTPD3 / NTPDase3 / CD39L3 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

ENTPD3 / NTPDase3 / CD39L3 Protein Description

Ectonucleoside triphosphate diphosphohydrolase 3, also known as ENTPD3, NTPDase3, and CD39L3, is an integral membrane glycoprotein. ENTPD3 is similar to E-type nucleotidases (NTPases). NTPasea, such as CD39, mediate catabolism of extracellular nucleotides. ENTPD3 contains 4 apyrase-conserved regions which is characteristic of NTPases. It has been reported that the obstructive disease cystic fibrosis (CF) affects the activity, expression, and tissue distribution of two ectonucleotidase found critical for the regulation of ATP on airway surfaces : NTPDase1 and NTPDase3. High densities of NTPDase3 positive terminals were associated with noradrenergic neurons. Semi-quantitative immunohistochemistry revealed increased NTPDase3 immunolabelling in the synaptic regions of the inner and outer hair cells at sound intensities that induce temporary threshold shift which suggest a role for NTPDase3 in regulating ATP signaling associated primarily with auditory neurotransmission, and the potential neuroprotective nature of noise-induced up-regulation of this extonucleotidase in the cochlea.

References

1. Fausther M. et al., 2010, Am J Physiol Lung Cell Mol Physiol. 298 (6): 804-18.
2. Vlajkovic SM. et al., 2006, Brain Res. 1104 (1): 55-63.
3. Ivanenkov w. et al., 2005, Biochemistry. 44 (25): 899890-12.