Anti-ENPP7 Antibody

Cat: 10885-RP02
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Anti-ENPP7 Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-ENPP7 Antibody
Validated applications
WB,ELISA
Species reactivity
Reacts with: Human
Specificity
Human ENPP7
Immunogen
Recombinant Human ENPP7 protein (Catalog#10885-H08H)
Preparation
Produced in rabbits immunized with purified, recombinant Human ENPP7 / NPP-7 (rh ENPP7; Catalog#10885-H08H; NP_848638.2; Met 1-Ser 439). ENPP7 specific IgG was purified by human ENPP7 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-ENPP7 Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:1000  
ELISA 1:5000-1:10000  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-ENPP7 Antibody (Rabbit Polyclonal antibody) Images
Human ENPP7 Western blot (WB) 13296

Anti-ENPP7 rabbit polyclonal antibody at 1:500 dilution

Lane A: HL-60 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:51 kDa

Observed band size:46 kDa

(We are unsure as to the identity of these extra bands.)

Anti-ENPP7 Antibody Alternative Names
Anti-ALK-SMase Antibody;Anti-E-NPP7 Antibody;Anti-NPP-7 Antibody;Anti-NPP7 Antibody
ENPP7 Background Information

Ectonucleotide pyrophosphatase / phosphodiesterase family member 7, also known as Alkaline sphingomyelin phosphodiesterase, Intestinal alkaline sphingomyelinase, Alk-Smase, ENPP7 and NPP-7, is a single-pass type I membrane protein which belongs to the nucleotide pyrophosphatase / phosphodiesterase family. ENPP7 / NPP-7 is expressed in the intestines and human bile. ENPP7 / NPP-7 is localized at the surface of the microvillar membrane in small intestine enterocytes, as well as in endosome-like structures and in Golgi complex. The main function of ENPP7 / NPP-7 is to convert the dietary sphingomyelin into ceramide, the sphingolipid messengers via hydrolyzation. ENPP7 / NPP-7 is also reported to exert a phospholipase C activity toward palmitoyl lyso-phosphocholine. The activity of this enzyme is inhibited in a dose dependent manner by ATP, imidazole, orthovanadate and zinc ion. Further, It has been shown in studies that decreased levels of ENPP7 / NPP-7 may be associated with human colon cancer.

Full Name
ectonucleotide pyrophosphatase/phosphodiesterase 7
References
  • Wu J. et al., 2005, Biochem. J. 386:153-60.
  • Wu J. et al., 2004, Carcinogenesis 25:1327-33.
  • Duan R.-D. et al., 2003, J. Lipid Res. 44:1241-50.
  • Duan R.-D. et al., 2003, J. Biol. Chem. 278:38528-36.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-ENPP7 Antibody Rabbit Polyclonal ELISA 10885-RP01
    Anti-ENPP7 Antibody Mouse Monoclonal ICC/IF,IF 10885-MM10
    Anti-ENPP7 Antibody Rabbit Monoclonal ELISA 10885-R001
    Anti-ENPP7 Antibody Mouse Monoclonal ELISA 10885-MM02
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