Anti-ENPP7 Antibody (Rabbit Polyclonal antibody) General Information
Reacts with: Human
Recombinant Human ENPP7 protein (Catalog#10885-H08H)
Produced in rabbits immunized with purified, recombinant Human ENPP7 / NPP-7 (rh ENPP7; Catalog#10885-H08H; NP_848638.2; Met 1-Ser 439). Total IgG was purified by Protein A affinity chromatography.
Polyclonal Rabbit IgG
0.2 μm filtered solution in PBS with 5% trehalose
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-ENPP7 Antibody (Rabbit Polyclonal antibody) Validated Applications
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
Anti-ENPP7 Antibody Alternative Names
Anti-ALK-SMase Antibody;Anti-E-NPP7 Antibody;Anti-NPP-7 Antibody;Anti-NPP7 Antibody
ENPP7 Background Information
Ectonucleotide pyrophosphatase / phosphodiesterase family member 7, also known as Alkaline sphingomyelin phosphodiesterase, Intestinal alkaline sphingomyelinase, Alk-Smase, ENPP7 and NPP-7, is a single-pass type I membrane protein which belongs to the nucleotide pyrophosphatase / phosphodiesterase family. ENPP7 / NPP-7 is expressed in the intestines and human bile. ENPP7 / NPP-7 is localized at the surface of the microvillar membrane in small intestine enterocytes, as well as in endosome-like structures and in Golgi complex. The main function of ENPP7 / NPP-7 is to convert the dietary sphingomyelin into ceramide, the sphingolipid messengers via hydrolyzation. ENPP7 / NPP-7 is also reported to exert a phospholipase C activity toward palmitoyl lyso-phosphocholine. The activity of this enzyme is inhibited in a dose dependent manner by ATP, imidazole, orthovanadate and zinc ion. Further, It has been shown in studies that decreased levels of ENPP7 / NPP-7 may be associated with human colon cancer.
ectonucleotide pyrophosphatase/phosphodiesterase 7
Wu J. et al., 2005, Biochem. J. 386:153-60. Wu J. et al., 2004, Carcinogenesis 25:1327-33. Duan R.-D. et al., 2003, J. Lipid Res. 44:1241-50. Duan R.-D. et al., 2003, J. Biol. Chem. 278:38528-36.