ENPP5 Protein (His Tag)

Ectonucleotide pyrophosphatase/phosphodiesterase 5 (putative) Protein Datasheet

ENPP5 Protein Product Information

• Synonym: ENPP5
• Protein Construction: A DNA sequence encoding the human ENPP5(Q9UJA9) (Met1-Gly429 )with a C-terminal polyhistidine tag was expressed.
• Source: Human
• Expression Host: Human Cells

ENPP5 Protein QC Testing

• Purity: > 96 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Pro 25
• Molecular Mass: The recombinant human ENPP5 comprises 416 amino acids and has a predicted molecular mass of 48.1 kDa. The apparent molecular mass of the protein is approximately 81 kDa in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: ENPP5 protein

ENPP5 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

ENPP5 Protein Description

ENPP5 belongs to the nucleotide pyrophosphatase/phosphodiesterase family(NPP) which catalyze the hydrolysis of phosphate diester bonds. NPP belongs to the alkaline phosphatase superfamily, which is a group of evolutionarily related enzymes that catalyze phosphoryl and sulfuryl transfer reactions. It is a family comprised by dimeric enzymes. There are seven isoforms in NPP family, some of which prefer nucleotide substrates, some of which prefer phospholipid substrates, and others of which prefer substrates that have not yet been determined. ENPP5 may play a role in neuronal cell communication. Hhowever, it lacks nucleotide pyrophosphatase and lysopholipase D activity. It may also be involved in neuronal cell communication. The amino acid sequence of human ENPP5 is 100%, 88%, and 82% identical to that of chimpanzee, dog and mouse/rat. It also belongs to a group of nucleotidemetabolizing ectoenzymes, which regulate the availability of extracellular nucleotides.

References

1. Strausberg RL. et al., 2002, Proc Natl Acad Sci. 99 (26): 16899-903.
2. Gerhard DS. et al., 2004, Genome Res. 14 (10B): 2121-7.
3. Lamesch P. et al., 2007, Genomics. 89 (3): 307-15.