Rat ENPP3 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
RG80366-G	Cloning vector ($295) pCMV / hygro ($545) pCMV / hygro - HA ($545) pCMV / hygro - His ($545) pCMV / hygro - Myc ($545) pCMV / hygro - FLAG ($545)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

ENPP3 cDNA Clone Datasheet

ENPP3 cDNA Clone Product Information

Gene Bank Ref.ID:	NM_019370.2
cDNA Size:	2619
Gene Synonym:	RB13-6; Enpp3
Source:	Rat

ENPP3 cDNA Clone Description

cDNA Description:	ORF Clone of Rattus norvegicus ectonucleotide pyrophosphatase/phosphodiesterase 3 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Same as Gene Bank Ref. ID sequence with the nucleotide 2620-2628 deletion mutation.

ENPP3 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

ENPP3 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

ENPP3 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGATTCCA GGCTAGCTTT AGCCACGGAG GAGCCCATTA AGAAAGACAG CCTGAAGAGA
61       TATAAGATCC TTTGTGCGGT TCTTCTTGCT TTGCTGGTGA TCGTGTCGCT TGGATTGGGC
121      CTGGGCCTGG GACTCAGGAA ACCTGAAGAG CATATAGGCA GCTGCAGGAA AAAATGCTTT
181      GATTCGTCAC ACAGAGGACT GGAGGGTTGC CGCTGTGACT CCGGATGCAC AGACCGGGGA
241      GACTGCTGCT GGGACTTTGA AGATACCTGT GTGAAATCAA CTCAAATCTG GACGTGTAAT
301      TCGTTCCGCT GTGGGGAGAC CAGACTAGAG GCAGCCCTCT GTTCCTGTGC GGATGACTGT
361      CTGCAGAGGA AGGACTGCTG CACTGACTAC AAGGCTGTGT GCCAAGGAGA AGTCCCATGG
421      GTGACAGAAG CCTGTGCCTC ATCCCAGGAG CCCCAGTGTC CAGAAGGGTT TGACCAGCCA
481      CCGGTTATCT TGTTCTCCAT GGACGGCTTT AGAGCGGAGT ACTTACAAAC ATGGAGCACC
541      CTGCTGCCAA ACATTAACAA ACTGAAAACA TGTGGACTCC ATTCCAAATA TATGAGAGCA
601      ATGTATCCCA CCAAAACCTT TCCAAATCAT TATACCATTG TCACGGGTTT GTATCCAGAG
661      TCGCACGGCA TCATTGACAA TAACATGTAT GATGTGTACC TCAACAAGAA TTTTTCACTT
721      TCTTCAGTGG AGAAAAGTAA CCCTGCCTGG TGGAGTGGGC AGCCGATTTG GTTAACAGCA
781      ATGTATCAAG GCTTAAAAGC TGCTTCCTAC TACTGGCCAG GATCAGATGT GGCTGTCAAT
841      GGCTCCTTCC CTAACATCTA CAGGAATTAC AGCAATTCCG TCCCATATGA AAGTAGGATT
901      GCTACACTGT TGCAATGGCT GGACCTGCCC AAAGCTGAAC GACCCAGTTT TTACACCATA
961      TATGTGGAAG AGCCCGATTC TGCGGGGCAC AAGAGTGGAC CAGTCAGTGC TGGAGTCATT
1021     AAAGCCTTAC AGTTAGTTGA TGATGCCTTT GGGATGCTGA TGGAGGGTTT GAAACAGAGG
1081     AATTTACACA ACTGTGTCAA CATAATCGTT CTGGCTGATC ATGGAATGGA CCAGACCTCT
1141     TGTGACAGAG TGGAATACAT GACAGATTAT TTTCCTGAAA TAAACTTCTA CATGTACCAA
1201     GGACCTGCCC CTCGAATCAG GACTCGTAAT ATACCTCAGG ACTTCTTTAC CTTTAATTCC
1261     GAAGAGATTG TCAGAGACCT CAGCTGTCGA AAATCCGATC AACACTTCAA ACCCTACTTG
1321     ACTCCGGATT TGCCGAAACG ACTGCACTAT GCCAAGAACG TCAGGATTGA CAAAGTTCAT
1381     CTAATGGTGG ATCGGCAATG GCTGGCTTAC AGGAACAAAG GGAGTTCCAA CTGCGAAGGC
1441     GGAACACACG GTTACAACAA TGAATTTAAA AGTATGGAGG CAATCTTTTT GGCACATGGA
1501     CCCAGCTTTA AAGAGAAGAC TGTTATCGAA CCATTTGAAA ACATCGAAGT CTATAATCTA
1561     CTGTGTGATC TTCTGCACAT CCAACCGGCA CCAAACAACG GTTCTCACGG TAGCCTGAAT
1621     CATCTTCTGA AGGCACCATT TTACCAGCCG TCGCATGCAG AGGAATTGTC AAAGTCTGCT
1681     GGCTGTGGTT TTACTACTCC ATTGCCTAAG GACTCACTAA ACTGCTCCTG CCTCGCACTG
1741     CAGACTTCTG GTCAAGAAGA GCAAGTCAAC CAAAGACTAA ATCTCAGTGG AGGAGAGGTC
1801     TCAGCAACAG AGAAGACAAA TTTGCCATTT GGGAGGCCCA GGGTGATACA GAAGAACAAA
1861     GACCACTGTC TGCTTTACCA CAGGGAATAC GTCAGTGGGT TTGGGAAAGC TATGAAGATG
1921     CCCATGTGGA GTTCATACAC CGTTCCCAAG CCGGGAGACA CATCATCTCT TCCTCCCACA
1981     GTTCCAGACT GTCTGCGGGC TGATGTCAGG GTTGATCCTT CGGAGAGCCA AAAATGTTCC
2041     TTCTATTTAG CAGACCAGAA TATCGACCAT GGCTTCCTCT ATCCTCCCGC AATCAAAGGA
2101     AATAATGAGA GTCAATATGA TGCTTTAATT ACAAGTAATT TGGTCCCAAT GTATAAAGAA
2161     TTCAAAAAAA TGTGGGACTA CTTCCATAAA GTTCTCCTTA TAAAATACGC TATAGAAAGA
2221     AATGGAGTGA ATGTGGTTAG TGGACCCATA TTTGATTACA ATTATGATGG CCATTTTGAT
2281     GCCCCCGATG AAATCACAAA CTATGTAGCT GGCACCGATG TTCCTGTCCC GACACACTAC
2341     TTTGTGGTAC TGACAAGCTG TAAAAACAAA ACCCACACAC CCGACAGCTG CCCTGGGTGG
2401     CTGGATGTCC TGCCCTTTGT CGTCCCTCAC CGGCCCACCA ACGTGGAAAG CTGTCCTGAA
2461     AACAAAGCAG AAGACCTTTG GGTTGAAGAG AGATTTAAAG CACACATCGC CCGGGTCCGT
2521     GATGTGGAGC TCCTCACTGG CCTTGACTTT TATCAGGAAA AAACACAGCC CGTCTCTGAA
2581     ATTTTGCAGC TGAAGACATA TTTGCCCACA TTCGAAACC

Quality Control:cDNA sequencing results is available upon request.