Mouse ENPP3 Gene cDNA Clone / ORF Clone

ENPP3 cDNA Clone Datasheet

ENPP3 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_134005.2
• cDNA Size: 2625
• Gene Synonym: CD203c; MGC6548; AI876438; Enpp3
• Source: Mouse

ENPP3 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus ectonucleotide pyrophosphatase/phosphodiesterase 3 DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence except for two point mutations:1427 A/G resulting in the amino acid asn substitution by ser, 597 T/A not causing the amino acid variation.

ENPP3 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

ENPP3 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

ENPP3 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGATTCCA GGCTAGCATT AGCCACAGAG GAGCCCATTA AGAAAGACAG TCTCAAGAAA
61       TACAAGATCC TTTGTGTGGT TCTTCTTGCT TTGTTGGTGA TTGTATCGCT TGGATTGGGG
121      CTGGGACTGG GACTCAGGAA ACCTGAAGAA CAAGGCAGCT GCAGGAAAAA ATGCTTTGAC
181      TCATCGCACA GAGGACTGGA GGGCTGCCGC TGTGACTCGG GATGCACAGG ACGAGGAGAC
241      TGCTGCTGGG ACTTTGAAGA TACCTGTGTG AAATCAACTC AAATATGGAC GTGCAATTTA
301      TTCCGTTGTG GGGAGAACAG ACTGGAGACA GCCCTCTGTT CCTGTGCGGA TGACTGTCTA
361      CAGAGGAAGG ACTGCTGTGC TGACTACAAG ACTGTGTGCC AAGGCGAAAG CCCATGGGTG
421      ACAGAAGCCT GTGCCTCATC CCAGGAGCCC CAGTGTCCAC CAGGGTTTGA CCTGCCACCA
481      GTTATCTTGT TCTCCATGGA CGGTTTTAGA GCTGAGTATT TACAAACATG GAGTACCCTT
541      TTGCCTAATA TCAACAAACT GAAAACATGT GGAATCCATT CTAAATATAT GAGAGCAATG
601      TATCCCACCA AAACCTTCCC AAATCATTAT ACCATTGTCA CGGGTTTGTA TCCGGAGTCA
661      CACGGCATCA TTGACAACAA CATGTATGAC GTGCACCTCA ACAAGAATTT TTCCCTTTCT
721      TCAGTGGAGA AAAGCAATCC CGCCTGGTGG AGCGGGCAGC CGATTTGGCT AACGGCAATG
781      TATCAAGGTT TAAAAGCTGC TTGCTACTAC TGGCCGGGAT CAGATGTGGC TGTCAATGGC
841      TCCTTCCCTA CCATCTACAG GAATTACAGC AATTCTGTCC CATATGAAAG GAGGATTACA
901      ACACTGTTAC AATGGCTGGA CCTGCCCAAA GCTGACCGAC CCAGTTTTTA TACCATCTAT
961      GTGGAAGAGC CTGATTCTGC AGGGCATTCG AGTGGACCAG TCAGTGCTGG AGTAATTAAA
1021     GCCTTGCAGT CAGTTGACAA TGCCTTTGGA ATGTTGATGG AGGGTCTGAA ACAGAGGAAT
1081     TTACACAACT GTGTCAATAT AATCGTTCTG GCTGATCATG GAATGGACCA GACCTCCTGT
1141     GATAGAGTGG AATACATGAC AGATTATTTT CCTAAAATAA ACTTCTATAT GTATCAAGGA
1201     CCTGCTCCTC GAATCAGGAC TCGTAATATA CCTCAGGACT TCTTTACATT TAATTCTGAA
1261     GAGATTGTCA GGAACCTCAG TTGTCGAAAA CCTGACCAAC ACTTTAAACC CTACTTGACC
1321     CCTGATTTGC CGAAACGACT GCACTACGCC AAAAATGTCA GAATTGACAA AGCTCATCTC
1381     ATGGTGGATC GGCAATGGCT GGCTTTCAGG AGCAAAGGGA GTTCCAGTTG TGGTGGAGGA
1441     ACACATGGCT ACAACAATGA GTTCAAGAGC ATGGAGGCAA TCTTTTTGGC ACATGGACCC
1501     AGCTTTATAG AGAAGACTGT TATTGAACCA TTTGAAAATA TTGAAGTCTA TAATCTACTG
1561     TGTGATCTTC TGCACATTGA ACCAGCACCA AACAACGGTA CTCATGGCAG TCTGAATCAT
1621     CTTCTGAAGA CACCGTTTTA CAAGCCATCA CATGCAGGAG AGTTGTCAAC GCCTGCTGAC
1681     TGTGGTTTTA CTACTCCCCT GCCTACAGAC CCACTAGACT GCTCATGCCC TGCACTACAG
1741     AATACGCCTG GTCTAGAAGA GCAAGCAAAC CAAAGGCTAA ATCTTAGCGA AGGCGAGGTT
1801     GCAGCAACGG TGAAAGCAAA TTTGCCATTT GGGAGGCCCA GGGTGATGCA GAAGAACGGA
1861     GACCACTGTC TCCTTTACCA CAGGGACTAC ATCAGTGGGT ACGGCAAAGC TATGAAGATG
1921     CCCATGTGGA GTTCATACAC CGTTCTCAAG CCGGGAGACA CATCATCTCT TCCTCCCACA
1981     GTTCCAGACT GTCTGCGGGC TGATGTCAGG GTTGCTCCTT CTGAGAGCCA AAAATGTTCC
2041     TTCTATTTAG CAGACAAGAA TATCACCCAT GGCTTCCTCT ATCCTGCAAT CAAAGGAACT
2101     AATGAGAGTC GATATGATGC CTTAATTACA AGTAATTTGG TCCCAATGTA TAAAGAATTC
2161     AAAAAAATGT GGGACTACTT CCATGAGGTT CTTCTTATAA AATATGCTAT AGAAAGAAAT
2221     GGACTGAATG TGGTTAGTGG ACCCATCTTT GATTATAATT ATGATGGCCA TTTTGATGCT
2281     CCGGATGAAA TCACACAATA TGTAGCTGGC ACTGATGTCC CTATCCCAAC ACACTACTTT
2341     GTGGTGCTGA CAAGTTGTAA AGACCAAACC CACACACCCG ACAGCTGCCC TGGGTGGCTG
2401     GATGTCCTGC CCTTCATCGT CCCTCACCGG CCCACCAACA TAGAAAGCTG TTCTGAAAAC
2461     AAGACCGAGG ACCTTTGGGT TGAAGAGAGG TTCCAAGCAC ACGCCGCCCG GGTCCGAGAT
2521     GTGGAGCTCC TCACTGGCCT TGACTTTTAT CAGGAGAAAG CACAGCCCGT CTCTCAGATT
2581     TTGCAACTAA AGACGTATCT GCCCACGTTT GAAACCATTA TTTGA

 

Quality Control:cDNA sequencing results is available upon request.