Rat ENPEP Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
RG80387-G	Cloning vector ($295) pCMV / hygro ($545) pCMV / hygro - HA ($545) pCMV / hygro - His ($545) pCMV / hygro - Myc ($545) pCMV / hygro - FLAG ($545)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

ENPEP cDNA Clone Datasheet

ENPEP cDNA Clone Product Information

Gene Bank Ref.ID:	NM_022251.2
cDNA Size:	2838
Gene Synonym:	Enpep
Source:	Rat

ENPEP cDNA Clone Description

cDNA Description:	ORF Clone of Rattus norvegicus glutamyl aminopeptidase DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutation 642 C/T not causing the amino acid variation.

ENPEP cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

ENPEP cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

ENPEP cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAACTTTG CAGAGGAAGA GCCCTCTAAG AAATACTGCA TTAAAGGCAA ACACGTGGCC
61       ATCATCTGCG CAACGGTGGT AGCAGTGGGA TTAATAGTAG GACTTTCTGT GGGCTTGACC
121      AGGTCGTGTG AGCCGGGCAC AACCCCCGCT CCTTCCAACC CTCCTCCCCA CACCAGCACT
181      GCCCTGCCTC CCCAGGATCA GAATGTCTGC CCTGACAGTG ACGATGAAAG TGGAGAATGG
241      AAAAACTTCA GGCTGCCGGA CTTCATCCAG CCAGTTCACT ACGACCTGGA GGTGAAGGTC
301      CTAATGGAGG AAGACAGGTA CACGGGCATA GTGAGCATCT CTGTCAACTT GAGCAAAGAC
361      ACTCGTGACC TATGGCTGCA CATCAGGGAA ACCAGGATCA CCAAGCTGCC GGAGCTAAGG
421      AGGCCCTCCG GGGAGCAGGT ACCGATTCGA CGGTGCTTTG AGTACAAGAA GCAGGAGTAC
481      GTGGTGATTC AGGCTGAAGA AGACCTTGCG GCCACCAGCG GGGACAGTGT CTATCGGCTG
541      ACCATAGAGT TTGAAGGCTG GTTGAACGGC TCCCTTGTGG GTTTTTACAG AACCACCTAC
601      ACGGAGGACG GGCAAACCAA GAGCATAGCG GCCACTGACC ATGAACCAAC AGATGCCAGG
661      AAGTCCTTCC CTTGTTTCGA TGAACCCAAC AAGAAGGCGA CTTACAACAT ATCCCTCATT
721      CACCCGAAAG AATACTCAGC ACTTTCCAAT ATGCCAGTAG AGAAGGAAGA GACACTGGAT
781      AACGACTGGA AGAAAACCAC TTTCATGAAG TCCGTCCCGA TGAGCACTTA CCTGGTGTGC
841      TTTGCTGTAC ATCAGTTCAC CTCGATACAG AGAACATCCA GGAGTGGCAA ACCACTCACT
901      GTTTACGTCC AGCCCAACCA AAAGCAAACA GCAGAGTATG CGGCAAACAT AACCAAAGCT
961      GTGTTTGACT TCTTTGAAGA CTACTTCGCT ATGGAGTATT CTCTTCCGAA ACTGGATAAA
1021     ATTGCTATTC CAGATTTTGG CACCGGCGCC ATGGAAAACT GGGGACTTGT CACTTACCGA
1081     GAAACAAACC TGCTTTATGA CCCCCTGCTA TCGGCCTCAT CCAACCAGCA GAGAGTAGCC
1141     AGCGTGGTTG CCCACGAGCT CGTGCACCAG TGGTTTGGAA ATATCGTGAC CATGGACTGG
1201     TGGGACGACT TGTGGCTAAA TGAAGGATTT GCTTCGTTCT TCGAGTTCTT GGGAGTAAAC
1261     CACGCAGAAG CAGACTGGCA GATGCTCAGT CAGGTGCTGC TTGAAGACGT GCTACCTGTG
1321     CAGGAGGATG ACTCCCTGAT GTCCTCACAT CCGGTGGTTG TCACGGTGTC TACTCCAGCT
1381     GAAATAACGT CTGTGTTCGA TGGAATATCA TACAGCAAGG GAGCTTCTAT CCTGAGAATG
1441     CTTCAAGACT GGATAACACC AGAGAAATTC CAAAAGGGGT GTCAGATTTA CTTGGAAAAC
1501     TTCAAGTTCA AGAACGCGAA AACTTCCGAC TTTTGGGATT CACTGGAAAA GGCAAGTAAT
1561     CAACCAGTGA AAGAAGTAAT GGACACCTGG ACTAGCCAGA TGGGCTATCC TGTGGTTACT
1621     GTGAGTGGAA AGCAGAACGT CACCCAGAAA CGCTTTCTGT TGGACTACAA AGCTGACCCT
1681     TCCCAGCCAC CGTCAGCACT TGGTTACACA TGGAATATTC CAATCAAATG GACCGAAAAT
1741     GGCAATTCAA ATATCACAGT CTACTATAGG TCAAACAGAG AAGGAATCAC TTTGAATGCT
1801     AATCTTAGCG GAGATGGTTT TCTTAAAATA AACCCAGATC ACATTGGGTT TTATCGTGTA
1861     AACTATGAAG CAGAAACTTG GGATTGGATA GCTGAGACTC TCTCCTCAAA CCACATGAAC
1921     TTCTCTTCTG CTGACCGGTC AAGTTTTATC GATGATGCTT TTGCTCTGGC AAGAGCTCAA
1981     CTTCTGGATT ATGAGAAGGC CCTGAACTTG ACCAGGTATC TCACATCAGA AAAGGATTTC
2041     TTACCATGGG AGAGAGTCAT TTCAGCTGTA AGCTACATCA TTAGCATGTT TGAAGATGAC
2101     AGAGAGCTGT ACCCCCTGAT AGAGACATAC TTCCGAAGCC AAGTGAAGCC CATTGCGGAT
2161     TCTCTGGGAT GGCAGGATAC CGGAAGCCAC ATCACAAAGT TACTCCGGGC CTCTGTATTA
2221     GGATTCGCAT GCAAGATGGG AGCCGGAGAA GCCTTGGGCA ATGCTTCCCA GTTATTTGAG
2281     GCCTGGCTGA AAGGGAATGA AAGCATTCCT GTGAACCTCA GGCTGCTGGT GTATCGCTAT
2341     GGAATGCAGA ACTCTGGCAA TGAGGCAGCG TGGAACTATA CTCTAGAGCA GTACCAGAAA
2401     ACATCTCTAG CACAAGAGAA AGAAAAGCTG CTCTATGGGT TAGCTTCGGT GAAGGATGTT
2461     ACACTCTTGG CAAGGTATCT GGAAATGCTC AAAGACCCCA ATATTATTAA AACTCAGGAC
2521     GTATTTACAG TCATCCGCTA CATCTCCTAC AACAGCTATG GGAAAAGCAT GGCCTGGAAC
2581     TGGATCCAAC TCAACTGGGA CTATCTGGTC AACAGATTTA CAATCAACGA CAGATACCTT
2641     GGCCGGATCG TCACCATAGC CGAGCCCTTC AACACTGAGC TGCAGCTCTG GCAGATGCAG
2701     AGCTTCTTTG CAAAATATCC AAATGCTGGC GCTGGAGCAA AACCCAGAGA GCAAGTGCTG
2761     GAAACGGTGA AGAACAACAT CGAGTGGCTA AAACTGAACA GAAAGTCCAT CAGCGAGTGG
2821     TTCACCAGCA TGCCCTAG

Quality Control:cDNA sequencing results is available upon request.