Human EMR3 Gene cDNA Clone / ORF Clone

EMR3 cDNA Clone Datasheet

EMR3 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_032571.3
• cDNA Size: 1959
• Gene Synonym: EMR3
• Source: Human

EMR3 cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens egf-like module containing, mucin-like, hormone receptor-like 3 DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 1154 G/A resulting in the amino acid Arg substitution by Gln.

EMR3 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

EMR3 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

EMR3 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCAGGGAC CATTGCTTCT TCCAGGCCTC TGCTTTCTGC TGAGCCTCTT TGGAGCTGTG
61       ACTCAGAAAA CCAAAACTTC CTGTGCTAAG TGCCCCCCAA ATGCTTCCTG TGTCAATAAC
121      ACTCACTGCA CCTGCAACCA TGGATATACT TCTGGATCTG GGCAGAAACT ATTCACATTC
181      CCCTTGGAGA CATGTAACGA CATTAATGAA TGTACACCAC CCTATAGTGT ATATTGTGGA
241      TTTAACGCTG TGTGTTACAA TGTCGAAGGA AGTTTCTACT GTCAATGTGT CCCAGGATAT
301      AGACTGCATT CTGGGAATGA ACAATTCAGT AATTCCAATG AGAACACCTG TCAGGACACC
361      ACCTCCTCAA AGACAACCGA GGGCAGGAAA GAGCTGCAAA AGATTGTGGA CAAATTTGAG
421      TCACTTCTCA CCAATCAGAC TTTATGGAGA ACAGAAGGGA GACAAGAAAT CTCATCCACA
481      GCTACCACTA TTCTCCGGGA TGTGGAATCG AAAGTTCTAG AAACTGCCTT GAAAGATCCA
541      GAACAAAAAG TCCTGAAAAT CCAAAACGAT AGTGTAGCTA TTGAAACTCA AGCGATTACA
601      GACAATTGCT CTGAAGAAAG AAAGACATTC AACTTGAACG TCCAAATGAA CTCAATGGAC
661      ATCCGTTGCA GTGACATCAT CCAGGGAGAC ACACAAGGTC CCAGTGCCAT TGCCTTTATC
721      TCATATTCTT CTCTTGGAAA CATCATAAAT GCAACTTTTT TTGAAGAGAT GGATAAGAAA
781      GATCAAGTGT ATCTGAACTC TCAGGTTGTG AGTGCTGCTA TTGGACCCAA AAGGAACGTG
841      TCTCTCTCCA AGTCTGTGAC GCTGACTTTC CAGCACGTGA AGATGACCCC CAGTACCAAA
901      AAGGTCTTCT GTGTCTACTG GAAGAGCACA GGGCAGGGCA GCCAGTGGTC CAGGGATGGC
961      TGCTTCCTGA TACACGTGAA CAAGAGTCAC ACCATGTGTA ATTGCAGTCA CCTGTCCAGC
1021     TTCGCTGTCC TGATGGCCCT GACCAGCCAG GAGGAGGATC CCGTGCTGAC TGTCATCACC
1081     TACGTGGGGC TGAGCGTCTC TCTGCTGTGC CTCCTCCTGG CGGCCCTCAC TTTTCTCCTG
1141     TGTAAAGCCA TCCAGAACAC CAGCACCTCA CTGCATCTGC AGCTCTCGCT CTGCCTCTTC
1201     CTGGCCCACC TCCTCTTCCT CGTGGGGATT GATCGAACTG AACCCAAGGT GCTGTGCTCC
1261     ATCATCGCCG GTGCTTTGCA CTATCTCTAC CTGGCCGCCT TCACCTGGAT GCTGCTGGAG
1321     GGTGTGCACC TCTTCCTCAC TGCACGGAAC CTGACAGTGG TCAACTACTC AAGCATCAAT
1381     AGACTCATGA AGTGGATCAT GTTCCCAGTC GGCTATGGCG TTCCCGCTGT GACTGTGGCC
1441     ATTTCTGCAG CCTCCTGGCC TCACCTTTAT GGAACTGCTG ATCGATGCTG GCTCCACCTG
1501     GACCAGGGAT TCATGTGGAG TTTCCTTGGC CCAGTCTGTG CCATTTTCTC TGCGAATTTA
1561     GTATTGTTTA TCTTGGTCTT TTGGATTTTG AAAAGAAAAC TTTCCTCCCT CAATAGTGAA
1621     GTGTCAACCA TCCAGAACAC AAGGATGCTG GCTTTCAAAG CAACAGCTCA GCTCTTCATC
1681     CTGGGCTGCA CATGGTGTCT GGGCTTGCTA CAGGTGGGTC CAGCTGCCCA GGTCATGGCC
1741     TACCTCTTCA CCATCATCAA CAGCCTCCAA GGCTTCTTCA TCTTCTTGGT CTACTGCCTC
1801     CTCAGCCAGC AGGTCCAGAA ACAATATCAA AAGTGGTTTA GAGAGATCGT AAAATCAAAA
1861     TCTGAGTCTG AGACATACAC ACTTTCCAGC AAGATGGGTC CTGACTCAAA ACCCAGTGAG
1921     GGGGATGTTT TTCCAGGACA AGTGAAGAGA AAATATTAA

 

Quality Control:cDNA sequencing results is available upon request.