Human EMILIN1 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13519-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

EMILIN1 cDNA Clone Datasheet

EMILIN1 cDNA Clone Product Information

Gene Bank Ref.ID:	BC007530
cDNA Size:	1044
Gene Synonym:	EMI;gp115;EMILIN；EMILIN1
Source:	Human

EMILIN1 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens elastin microfibril interfacer 1 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

EMILIN1 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

EMILIN1 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

EMILIN1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCTGACC TGGGGGCAAC CAAGGACCGT ATCATTTCTG AGATTAACAG GCTGCAGCAG
61       GAGGCCACAG AGCATGCTAC AGAGAGTGAA GAGCGCTTCC GAGGCCTAGA GGAGGGACAA
121      GCACAGGCCG GCCAGTGCCC CAGCTTAGAG GGGCGATTGG GCCGTCTTGA GGGTGTCTGT
181      GAACGGTTGG ACACTGTGGC TGGGGGACTG CAGGGCCTGC GCGAGGGCCT TTCCAGACAC
241      GTGGCTGGGC TCTGGGCTGG GCTCCGGGAA ACCAACACCA CCAGCCAGAT GCAGGCAGCC
301      CTGCTGGAGA AGCTGGTCGG GGGACAGGCG GGCCTGGGCA GGCGGCTGGG TGCCCTTAAC
361      AGCTCCCTGC AGCTCCTGGA GGACCGTCTG CACCAGCTCA GCCTGAAGGA CCTCACTGGT
421      GAGGGGACAA AAGGGCCTGC AGGAGAGGCT GGGCCCCCAG GGCCTCCTGG GCTGCAGGGA
481      CCCCCAGGCC CTGCTGGACC TCCAGGATCA CCAGGCAAGG ACGGGCAAGA GGGCCCCATC
541      GGGCCACCAG GTCCTCAAGG TGAACAGGGA GTGGAGGGGG CACCAGCAGC CCCTGTGCCC
601      CAAGTGGCAT TTTCAGCTGC TCTGAGTTTG CCCCGGTCTG AACCAGGCAC GGTCCCCTTC
661      GACAGAGTCC TGCTCAATGA TGGAGGCTAT TATGATCCAG AGACAGGCGT GTTCACAGCG
721      CCACTGGCTG GACGCTACTT GCTGAGCGCG GTGCTGACTG GGCACCGGCA CGAGAAAGTG
781      GAGGCCGTGC TGTCCCGCTC CAACCAGGGC GTGGCCCGCG TAGACTCCGG TGGCTACGAG
841      CCTGAGGGCC TGGAGAATAA GCCGGTGGCC GAGAGCCAGC CCAGCCCGGG CACCCTGGGC
901      GTCTTCAGCC TCATCCTGCC GCTGCAGGCC GGGGACACGG TCTGCGTCGA CCTGGTCATG
961      GGGCAGCTGG CGCACTCGGA GGAGCCGCTC ACCATCTTCA GCGGGGCCCT GCTCTATGGG
1021     GACCCAGAGC TTGAACACGC GTAG

Quality Control:cDNA sequencing results is available upon request.