Human EMB Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13704-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

EMB cDNA Clone Datasheet

EMB cDNA Clone Product Information

Gene Bank Ref.ID:	BC059398
cDNA Size:	984
Gene Synonym:	EMB
Source:	Human

EMB cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens embigin homolog (mouse) DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

EMB cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

EMB cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

EMB cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCGCGCCC TCCCCGGCCT GCTGGAGGCC AGGGCGCGTA CGCCCCGGCT GCTCCTCCTC
61       CAGTGCCTTC TCGCTGCCGC GCGCCCAAGC TCGGCGGACG GCAGTGCCCC AGATTCGCCT
121      TTTACAAGTC CACCTCTCAG AGAAGAAATA ATGGCAAATA ACTTTTCCTT GGAGAGTCAT
181      AACATATCAC TGACTGAACA TTCTAGTATG CCAGTAGAAA AAAATATCAC TTTAGAAAGG
241      CCTTCTAATG TAAATCTCAC ATGCCAGTTC ACAACATCTG GGGATTTGAA TGCAGTAAAT
301      GTGACTTGGA AAAAAGATGG TGAACAACTT GAGAATAATT ATCTTGTCAG TGCAACAGGA
361      AGCACCTTGT ATACCCAATA CAGGTTCACC ATCATTAATA GCAAACAAAT GGGAAGTTAT
421      TCTTGTTTCT TTCGAGAGGA AAAGGAACAA AGGGGAACAT TTAATTTCAA AGTCCCTGAA
481      CTTCATGGGA AAAACAAGCC ATTGATCTCT TACGTAGGGG ATTCTACTGT CTTGACATGT
541      AAATGTCAAA ATTGTTTTCC TTTAAATTGG ACCTGGTACA GTAGTAATGG GAGTGTAAAG
601      GTTCCTGTTG GTGTTCAAAT GAATAAATAT GTGATCAATG GAACATATGC TAACGAAACA
661      AAGCTGAAGA TAACACAACT TTTGGAGGAA GATGGGGAAT CTTACTGGTG CCGTGCACTA
721      TTCCAATTAG GCGAGAGTGA AGAACACATT GAGCTTGTGG TGCTGAGCTA TTTGGTGCCC
781      CTCAAACCAT TTCTTGTAAT AGTGGCTGAG GTGATTCTTT TAGTGGCCAC CATTCTGCTT
841      TGTGAAAAGT ACACACAAAA GAAAAAGAAG CACTCAGATG AGGGGAAAGA ATTTGAGCAG
901      ATTGAACAGC TGAAATCAGA TGATAGCAAT GGTATAGAAA ATAATGTCCC CAGGCATAGA
961      AAAAATGAGT CTCTGGGCCA GTGA

Quality Control:cDNA sequencing results is available upon request.