ELK1 Protein (GST Tag)

Member of ETS oncogene family Protein Datasheet

ELK1 Protein Product Information

• Synonym: RP23-275N2.2, Elk-1
• Protein Construction: A DNA sequence encoding the human ELK1 (P19419-1) (Met 1-Pro 428) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
• Source: Human
• Expression Host: Baculovirus-Insect cells

ELK1 Protein QC Testing

• Purity: > 85 % as determined by SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant human ELK1/GST chimera consists of 665 amino acids and migrates as an approximately 73 KDa band in SDS-PAGE under reducing conditions as predicted.
• Formulation: Lyophilized from 0.2μm filtered solution of 20mM Tris, 500mM NaCl, pH7.4, 10% gly
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: ELK1 protein

ELK1 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

ELK1 Protein Description

Member of ETS oncogene family (ELK1) is a member of the ETS oncogene superfamily which is characterized by a common protein domain that regulates DNA binding to target sequences. The Elk1 protein is composed of several domains. The A domain is restricted in the N-terminal region. It’s required for the binding of Elk1 to DNA. The B domain allows Elk1 to bind to a dimer of its cofactor, serum response factor(SRF). The C domain is located in the C-terminal region of Elk1. It includes the amino acids that is phosphorylated by MAPKs. The D domain plays a important role of binding to active MAPKs. The R domain is located adjacent to the B domain that is involved in suppressing Elk1 transcriptional activity. This domain harbors the lysine residues that undergo SUMOylation, a post-translational event that strengthens the inhibition function of the R domain. Finally, the DEF domain is specific for the interaction of activated extracellular signal-regulated kinase (Erk), a type of MAPK, with Elk1. Elk1 plays important roles in various contexts, including long-term memory formation, drug addiction, Alzheimer’s disease, Down syndrome, breast cancer, and depression.

References

1. Shin SY. et al., 2011, J Biol Chem. 286 (30): 26860-72.
2. Taniue K. et al., 2011, EMBO Rep. 12 (7): 682-9.
3. Pan HF. et al., 2011, Lupus. 20 (3): 227-30.