Mouse ELA2 Gene cDNA Clone / ORF Clone

ELA2 cDNA Clone Datasheet

ELA2 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_015779.2
• cDNA Size: 798
• Gene Synonym: NE; F430011M15Rik; Ela2
• Source: Mouse

ELA2 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus elastase 2, neutrophil DNA.
• Vector: Vector pMD18-T Simple
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

ELA2 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

ELA2 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

ELA2 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCCCTTG GCAGACTATC CAGCCGGACT CTGGCTGCCA TGCTACTGGC ATTGTTCCTG
61       GGTGGCCCAG CACTGGCCTC AGAGATTGTT GGTGGCCGGC CGGCCCGGCC CCATGCTTGG
121      CCCTTCATGG CATCCCTGCA GAGGCGTGGA GGTCATTTCT GTGGTGCCAC CCTCATTGCC
181      AGGAACTTCG TCATGTCAGC AGCCCACTGT GTGAACGGCC TAAATTTCCG GTCAGTGCAG
241      GTAGTGCTGG GAGCCCATGA CCTGCGGCGA CAGGAGCGCA CTCGACAGAC CTTCTCTGTG
301      CAGCGGATCT TCGAGAATGG CTTTGACCCA TCACAACTGC TGAACGACAT TGTGATTATC
361      CAGCTCAATG GCTCCGCTAC CATTAACGCC AACGTGCAGG TGGCCCAGCT GCCTGCCCAG
421      GGCCAGGGCG TGGGTGACAG AACTCCATGT CTGGCCATGG GCTGGGGCAG GTTGGGCACA
481      AACAGACCAT CACCCAGTGT GCTACAAGAG CTCAATGTGA CAGTGGTGAC TAACATGTGC
541      CGCCGTCGTG TGAACGTATG CACTCTGGTG CCACGTCGGC AGGCAGGCAT CTGCTTCGGG
601      GACTCTGGCG GACCCTTGGT CTGTAACAAC CTTGTCCAAG GCATTGACTC CTTCATCCGA
661      GGAGGCTGTG GATCTGGATT GTACCCAGAT GCCTTCGCCC CTGTGGCTGA GTTTGCAGAT
721      TGGATCAATT CCATTATCCG AAGCCATAAT GACCACCTTC TTACCCATCC CAAAGACCGA
781      GAGGGCAGGA CCAACTAG

 

Quality Control:cDNA sequencing results is available upon request.