EIF-5A / EIF5 Protein (GST Tag)

Eukaryotic translation initiation factor 5 Protein Datasheet

EIF-5A / EIF5 Protein Product Information

• Synonym: EIF5
• Protein Construction: A DNA sequence encoding the mature form of human EIF5 (P55010) (Met1-Asp150) was fused with the GST tag at the N-terminus.
• Source: Human
• Expression Host: E.coli

EIF-5A / EIF5 Protein QC Testing

• Purity: > 95 % as determined by SDS-PAGE
• Endotoxin: Please contact us for more information.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant human EIF5 /GST chimera consists of 384 amino acids and has a predicted molecular mass of 44.1 kDa. It migrates as an approximately 38-43 KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: EIF-5A / EIF5 protein

EIF-5A / EIF5 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

EIF-5A / EIF5 Protein Description

EIF-5A, also known as EIF5, plays an important role in the assembly of the 43S pre-initiation complex. In this assembly, EIF-5A interacts with eIF1 and eIF3 and binds the eIF2-GTP/Met-tRNA ternary complex along with the 40S ribosome subunit. EIF-5A is also reported to form a complex with eIF2. It is a critical component of the eIF2regulatory complex that inhibits the activity of the guanine-nucleotide exchange factor (GEF) eIF2B. This complex is suggested to serve as a cytoplasmic reservoir for eIF2 that restricts the guanine nucleotide exchange promoted by the eIF2 beta subunit for the initiation of translation.

References

1. Si K. et al., 1996, J Biol Chem. 71 (28): 16934-8.
2. Das S. et al., 1998, J Biol Chem. 272 (50): 31712-8.
3. Conte MR. et al., 2006, Biochemistry. 45 (14): 4550-8.