All EIF5 reagents are produced in house and quality controlled, including 1 EIF5 Antibody, 14 EIF5 Gene, 1 EIF5 Protein, 1 EIF5 qPCR. All EIF5 reagents are ready to use.
Recombinant EIF5 proteins are expressed by E. coli with fusion tags as N-GST.
EIF5antibodies are validated with different applications, which are ELISA, IHC-P, ICC/IF, IF.
EIF5cDNA clones are full length sequence confirmed and expression validated. There are 13 kinds of tags for each EIF5 of different species, especially GFP tag, OFP tag, FLAG tag and so on. There are three kinds of vectors for choice, cloning vector, expression vector and lentivrial expression vector.
EIF-5A, also known as EIF5, functions in start site selection as a GTPase accelerating protein (GAP) for the eukaryotic translation initiation factor (eIF) 2•GTP•tRNA ternary complex within the ribosome-bound pre-initiation complex. In protein synthesis initiation, eIF2 functions in its GTP-bound state to deliver initiator methionyl-tRNA to the small ribosomal subunit and is necessary for protein synthesis in all cells. EIF-5A stabilizes the binding of GDP to eIF2 and is therefore a bi-functional protein that acts as a GDP dissociation inhibitor (GDI). EIF-5A also interacts with eIF1 and eIF3 and binds the eIF2-GTP/Met-tRNA ternary complex along with the 40S ribosome subunit.