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Canine EGFR Gene cDNA Clone / ORF Clone
| Catalog | Vector (Price) | Quantity | In Stock | Operation |
| DG70026-G |
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YES |  |
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* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. |
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EGFR cDNA Clone Datasheet
EGFR cDNA Clone Product Information
| Gene Bank Ref.ID: | XM_533073.3 |
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cDNA Size: |
3543 |
| Gene Synonym: |
EGFR |
| Source: | Canine |
EGFR cDNA Clone Description
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cDNA Description: |
ORF Clone of Canis lupus familiaris epidermal growth factor receptor DNA. |
| Vector: | Cloning Vector: pGEM-T Vector
Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG |
| Sequence Description: | Identical with the Gene Bank Ref. ID sequence except for the point mutation 1258 T/C, resulting in the amino acid Ser substitution by Pro. |
EGFR cDNA Clone Shipping
- 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
- It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.
EGFR cDNA Clone Usage Protocol
- Cut along the circle edge of the spot (arrow points to) to get the filter paper.
- Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O Ensure the filter is completely immersed in the H2O by briefly centrifuging the tube for 10 seconds.
- Transfer the tube to incubation at 95℃ for 15-30 min.
- At the end of the incubation pulse vortex, or gently tap the tube.
- Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.
EGFR cDNA Clone Sequence and Quality Control
Sequence:
1 ATGGCGGTCT GCCAAGGCAC GAGTAACCGG CTCACCCAGT TGGGCACTTT TGAAGACCAC 61 TTTCTGAGCC TTCAGAGGAT GTTCAATAAC TGTGAGGTGG TCCTGGGGAA TTTGGAAATT 121 ACCTACATGC AAAGGAATTA TGACCTTTCC TTCTTAAAGA CCATCCAGGA AGTTGCCGGG 181 TATGTATTGA TTGCCCTCAA CACGGTGGAG AAGATTCCTT TGGAAAACCT GCAGATCATC 241 CGAGGAAATG TGCTCTATGA AAACACCCAT GCCTTATCCG TCCTCTCCAA CTATGGCTCA 301 AATAAAACTG GACTGCAGGA GCTTCCCCTG AGAAACTTGC ATGAAATACT ACAAGGCGCT 361 GTGCGCTTCA GCAATAACCC TGTCCTCTGC AACGTGGAAA CCATCCAGTG GCGGGACATC 421 GTTGACAACG ATTTTATAAG CAACATGTCG ATGGACATCC AGAACCAAGC GGGCAGGTGC 481 CAAAAGTGTG ATCCAAGCTG TCCCAATGGA AGTTGCTGGG GTCCGGGAAA GGAGAATTGC 541 CAGAAACTGA CCAAAATCAT CTGTGCCCAG CAGTGTTCGG GGCGCTGCCG CGGCAGGTCC 601 CCCAGCGACT GCTGCCACAA CCAGTGTGCC GCCGGCTGCA CGGGGCCCCG CGAGAGCGAC 661 TGCCTGGTCT GCCGCAAGTT CCGGGATGAA GCCACGTGCA AGGACACCTG CCCGCCCCTC 721 ATGCTCTACA ACCCCACCAC CTACCAAATG GACGTCAACC CAGAGGGAAA ATACAGCTTT 781 GGTGCCACCT GCGTGAAGAA ATGCCCCCGC AACTACGTGG TGACAGACCA CGGTTCATGT 841 GTCCGCGCCT GCAGCTCTGA CAGCTACGAG GTGGAGGAGG ATGGTGTCCG CAAGTGTAAG 901 AAGTGTGAGG GGCCTTGTCG CAAAGTTTGT AATGGAATAG GGATTGGAGA GTTCAAAGAC 961 ACACTTTCCA TAAATGCTAC AAACATTAAA CACTTCAAAA ACTGCACGTC GATCAGTGGA 1021 GACCTTCATA TCTTGCCAGT CGCATTTAGA GGTGACTCCT TCACGCATAC CCTACCTCTA 1081 GATCCGAAGG AGCTGGATAT CCTGAAAACT GTCAAGGAAA TAACAGGGTT TTTGCTGATT 1141 CAGGCCTGGC CCGAAAATAG GACTGACCTC CATGCTTTCG AGAACCTAGA AATCATACGT 1201 GGAAGAACAA AGCAACATGG CCAGTTTTCT CTGGCGGTCG TCGGCCTGAA CATAACGCCC 1261 TTGGGATTAC GCTCCCTCAA GGAGATAAGC GATGGAGATG TGATCATCTC TGGAAACCGA 1321 AAATTGTGCT ATGCAAATAC AATAAACTGG AAGAAACTCT TTGGGACTTC AAGTCAGAAA 1381 ACCAAAATTA TAAACAACAA AGATGAAAAA GCCTGCAAGG CCATAGGCCA CGTCTGCCAT 1441 CCATTGTGCT CCTCAGAAGG CTGCTGGGGC CCAGGGCCGA GAGACTGCGT GTCCTGCCGA 1501 AACGTCAGCC GTGGCAAGGA ATGTGTGGAA AAGTGCAACA TTCTGGAGGG GGAGCCAAGG 1561 GAGTTTGTGG AGAATTCGGA GTGCATACAG TGCCATCCGG AATGCCTGCC CCAGGCCATG 1621 AACATAACCT GCACAGGACG GGGGCCGGAC AGCTGCATCA AGTGCGCCCA CTACATCGAT 1681 GGCCCTCACT GCGTCAAGAC CTGCCCGGCT GGCATCATGG GAGAAAACAA CACCCTGGTC 1741 TGGAAGTTTT CGGATGGCAG CCGCATGTGC CACCTGTGCC ATCCAAACTG CACCTATGGC 1801 TGTGAGGGAC CAGGTCTTGA AGGCTGTGCA AAACCTGGGC CCAAGATCCC ATCCATTGCT 1861 ACCGGGATTG TCGGCGGCCT CCTCTTGGTG GTGGTGGTGG CCCTTGGAGT GGGCCTCTTT 1921 TTGCGCCGGC GCCACATTGT CCGGAAGCGC ACGCTTCGCA GACTGCTGCA AGAAAGAGAG 1981 CTTGTCGAGC CTCTTACACC CAGCGGAGAA GCTCCCAACC AGGCTCTCTT GAGGATCTTA 2041 AAGGAGACGG AGTTCAAAAA GATCAAGGTG CTGGGCTCTG GAGCATTCGG CACAGTGTAC 2101 AAGGGACTCT GGATCCCAGA AGGCGAGAAG GTTAAAATTC CCGTGGCCAT CAAGGAATTG 2161 AGAGAAGCCA CGTCTCCCAA AGCCAACAAG GAAATTCTTG ATGAAGCCTA CGTGATGGCC 2221 AGTGTGGACA ATCCCCACGT GTGCCGCCTC CTGGGCATCT GCCTGACGTC CACGGTGCAG 2281 CTCATCACGC AGCTCATGCC CTTTGGCTGC CTCCTGGACT ATGTCCGCGA GCACAAGGAC 2341 AACATCGGCT CCCAGCACCT GCTCAACTGG TGTGTGCAGA TTGCAAAGGG CATGAACTAC 2401 CTGGAAGACC GGCGCTTGGT GCACCGCGAC CTGGCGGCCA GGAACGTCCT GGTGAAGACC 2461 CCGCAGCACG TCAAGATCAC AGATTTTGGG CTGGCCAAAC TGCTGGGTGC CGAGGAGAAA 2521 GAGTACCACG CGGAAGGAGG CAAAGTGCCT ATCAAGTGGA TGGCTTTAGA ATCGATTTTA 2581 CACCGAATTT ATACCCACCA AAGCGATGTG TGGAGCTACG GCGTCACCGT GTGGGAGCTG 2641 ATGACCTTCG GGTCCAAGCC TTACGACGGT ATCCCTGCAA GTGAGATCTC CACCATCCTG 2701 GAGAAGGGAG AGCGCCTCCC GCAGCCGCCC ATATGCACCA TCGATGTCTA CATGATCATG 2761 GTCAAGTGCT GGATGATAGA TGCAGACAGT CGCCCAAAAT TCCGTGAGTT GATCATCGAA 2821 TTCTCCAAAA TGGCCCGAGA CCCGCAGCGC TACCTTGTCA TCCAGGGAGA TGAGAGGATG 2881 CATTTGCCAA GCCCTACAGA CTCCAATTTT TACCGCGCCC TGATGGACGA GGAGGACATG 2941 GAGGATGTTG TGGATGCTGA CGAGTACCTC ATCCCCCAGC AGGGCTTCTT CCACAGCCCC 3001 TCCACTTCCC GGACCCCCCT CCTAAGTTCT CTGAGCGCCA CCAGCAACAG TTCCAACGTG 3061 GCTTGCATCG ACCGAAATGG GACCTGTCCC CTCAAAGAAG ACAGCTTCTT GCAGCGGTAC 3121 AGCTCAGACC CCACTGGCAC CTTGACGGAG GACAACATAG ATGACACTTT CCTCCCAGCA 3181 CCCGAATACA TAAACCAGTC TGTTCCCAAA AGGCCTGCGG GTTCTGTCCA GAATCCCGTC 3241 TATCACAATC AGCCTCTAAA TCCAGCTCCT GCCAGAGACC CTCACTACCA AAATCCCCAC 3301 AGCAACGCAG TGGACAACCC TGAGTATCTC AACACCCACC CCACCTGCGT CAACAGTGTC 3361 CTCGACAGGC CCAGCCTCTG GACCCAGGAG GCCAACCACC AAATCAGCCT GGACAACCCT 3421 GACTACCAGC AGGACTTCTT TCCCAAGGAA GCCAAGTCCA ATGGCATTTT TAAGGGCCCT 3481 GCAGCTGAAA ATGCAGACTA CCTGAGGGTA GCGCCACCAA GCAGTGAGTT TATTGGAGCG 3541 TGA
Quality Control:cDNA sequencing results is available upon request.
