|Datasheet||Specific References||Reviews||Related Products||Protocols|
|ORF Clone of Mus musculus epidermal growth factor receptor DNA.|
|Wa5, wa2, Erbb, Errp, wa-2, AI552599, 9030024J15Rik, Egfr|
|Identical with the Gene Bank Ref. ID sequence except for the point mutation 40 T/C, 1725 A/G and 2799 G/A not causing the amino acid variation.|
|Whatman FTA elute card (Cat: WB120410) contains 5-10 μg of plasmid.|
|The Whatman FTA elute card can be stored at room temperature for three months under dry condition.|
The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.
|Mouse EGFR Gene cDNA Clone (full-length ORF Clone), expression ready, FLAG-tagged||MG51091-G-F|
|Mouse EGFR Gene cDNA Clone (full-length ORF Clone), expression ready, His-tagged||MG51091-G-H|
|Mouse EGFR Gene cDNA Clone (full-length ORF Clone), expression ready, Myc-tagged||MG51091-G-M|
|Mouse EGFR Gene cDNA Clone (full-length ORF Clone), expression ready, untagged||MG51091-G-N|
|Mouse EGFR Gene cDNA Clone (full-length ORF Clone), expression ready, HA-tagged||MG51091-G-Y|
|Product name||Product name|
As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.