|Datasheet||Specific References||Reviews||Related Products||Protocols|
|ORF Clone of Homo sapiens epidermal growth factor receptor DNA.|
|EGFR, ERBB, HER1, mENA, ERBB1, PIG61|
|KpnI + XhoI|
|Identical with the Gene Bank Ref. ID sequence except for the point mutation 2361 G/A not causing the amino acid variation.|
|Whatman FTA elute card (Cat: WB120410) contains 5-10 μg of plasmid.|
|The Whatman FTA elute card can be stored at room temperature for three months under dry condition.|
The Vector 3 is 5.4kb in length, and contains the the amplicin resistance gene, conferring selection of the plasmid in E. coli., the kanamycin resistance gene (neomycin for mammalian selection), and the CMV promoter upstream of the cDNA insert. The SV40 origin allows for replication in mammalian cells, the ColE1 origin is the bacterial origin of replication, and the f1 origin is the filamentous phage origin of replication. The plasmid has multiple cloning sites as shown below. NOTE: the restriction enzymes used in each vector 3 clone is indicated in the product datasheet.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with primer pair SP6 and T7.
|Human EGFR Gene cDNA Clone (full-length ORF Clone), expression ready, FLAG-tagged||HG10001-C-F|
|Human EGFR Gene cDNA Clone (full-length ORF Clone), expression ready, His-tagged||HG10001-C-H|
|Human EGFR Gene cDNA Clone (full-length ORF Clone), expression ready, Myc-tagged||HG10001-C-M|
|Human EGFR Gene cDNA Clone (full-length ORF Clone), expression ready, untagged||HG10001-C-N|
|Human EGFR Gene cDNA Clone (full-length ORF Clone), expression ready, HA-tagged||HG10001-C-Y|
|Product name||Product name|
As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.