Anti-EGFR Antibody

Cat: 10001-RE10
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Anti-EGFR Antibody (Rabbit Monoclonal antibody) General Information
Product name
Anti-EGFR Antibody
Validated applications
Neutralization
Species reactivity
Reacts with: Human
Specificity
Human EGFR
Has cross-reactivity with Mouse EGFR (Catalog # 951091-M08H) , Rat EGFR (Catalog # 80100-R08H) and Rhesus EGFR (Catalog # 90317-K08H) in ELISA assay
Immunogen
Recombinant Human EGFR / HER1 / ErbB1 Protein (Catalog#10001-H08H)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human EGFR / HER1 / ErbB1 (rh EGFR / HER1 / ErbB1; Catalog#10001-H08H; NP_005219; Met1-Ser645) and was produced using recombinant antibody technology.
Source
Monoclonal Rabbit IgG Clone #RE10
Purification
Protein A
Formulation
0.2 μm filtered solution in Histidine and Arginine buffer containing 120mM NaCl, 0.02% Tween 80, pH6.0
Conjugate
Unconjugated
Endotoxin
< 3 EU/mg
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-EGFR Antibody (Rabbit Monoclonal antibody) Validated Applications
Application Notes
Block In a functional ELISA which immobilized the recombinant Human EGF (Catalog # 10605-H01H) at 2 μg/mL (100 μl/well) , 20 μg/mL of the Human EGFR antibody (Catalog # 10001-RE10) can blocked > 50% the binding of recombinant receptor EGFR (Catalog # 10001-H08H) at 0.08 μg/mL
Neutralization The neutralization activity of EGFR Neutralizing Antibody is Measured by its ability to neutralize autocrine EGF induced proliferation in the human breast cancer MDA-MB-468 cell line. The Neutralization titer (IC50) is typically 0.05-0.2 μg/mL for 5000 cells/well.

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-EGFR Antibody (Rabbit Monoclonal antibody) Images
Human EGFR/HER1 Blocking/Neutralization (B/N) 5602
Cell Proliferation Induced by Autocrine EGF was Neutralized by Human EGFR Antibody. Autocrine EGF stimulates proliferation in the human breast cancer MDA-MB-468 cell line. Proliferation elicited by Autocrine EGF is neutralized by increasing concentrations of Human EGFR Monoclonal Antibody (Catalog # 10001-RE10). The IC50 is typically 0.05-0.2 μg/mL.
Anti-EGFR Antibody Alternative Names
Anti-ERBB Antibody;Anti-ERBB1 Antibody;Anti-HER1 Antibody;Anti-mENA Antibody;Anti-NISBD2 Antibody;Anti-PIG61 Antibody
EGFR Background Information

As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.

Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Full Name
epidermal growth factor receptor
References
  • Schlessinger, J. (2000) Cell signaling by receptor tyrosine kinases. Cell 103(2): 211-25.
  • Giaccone, G. (2005) HER1/EGFR-targeted agents: predicting the future for patients with unpredictable outcomes to therapy. Ann. Oncol. 16(4): 538-48.
  • Yarden, Y., et al. (2001) Untangling the ErbB signalling network. Nat. Rev. Mol. Cell. Biol. 2(2): 127-37.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-EGFR Antibody Rabbit Polyclonal ELISA 90317-T16
    Anti-EGFR Antibody Rabbit Polyclonal ELISA 90317-RP01
    Anti-EGFR Antibody Rabbit Polyclonal WB,ELISA,IP 80100-T52
    Anti-EGFR Antibody Rabbit Polyclonal ELISA 80100-RP01
    Anti-EGFR Antibody Rabbit Polyclonal WB,ELISA,IP 51091-T52
    Anti-EGFR Antibody Rabbit Monoclonal ELISA 51091-R112
    Anti-EGFR Antibody Rabbit Polyclonal WB,ELISA 51091-T48
    Anti-EGFR Antibody Rabbit Monoclonal ELISA 51091-R004
    Anti-EGFR Antibody Rabbit Polyclonal ELISA 51091-RP01
    Anti-EGFR Antibody Rabbit Monoclonal WB,ELISA,ELISA(Det),ICC/IF,IF 10001-R021
    Anti-EGFR Antibody Rabbit Polyclonal ELISA 10001-RP02
    Anti-EGFR Antibody Mouse Monoclonal ELISA 10001-MM01
    Anti-EGFR Antibody (APC) Mouse Monoclonal FCM 10001-MM08-A
    Anti-EGFR Antibody (APC) Rabbit Monoclonal FCM 10001-RE01-A
    Anti-EGFR Antibody Rabbit Polyclonal ELISA,IHC-P 10001-T24
    Anti-EGFR Antibody Rabbit Polyclonal ELISA 10001-RP01
    Anti-EGFR Antibody Rabbit Polyclonal WB 100372-T32
    Anti-EGFR Antibody (PE) Mouse Monoclonal FCM 10001-MM08-P
    Anti-EGFR Antibody Rabbit Monoclonal ELISA,FCM 10001-RE01
    Anti-EGFR Antibody Rabbit Monoclonal ELISA,IHC-P,IHC-Fr 10001-R043
    Anti-EGFR Antibody Mouse Monoclonal FCM,ICC/IF,IF 10001-MM08
    Anti-EGFR Antibody Mouse Monoclonal IHC-P 10001-MM11
    Anti-EGFR Antibody Rabbit Monoclonal ELISA(Cap) 10001-RE11
    Anti-EGFR Antibody (PE) Rabbit Monoclonal FCM 10001-RE01-P
    Anti-EGFR Antibody (FITC) Rabbit Monoclonal FCM 10001-RE01-F
    Anti-EGFR Antibody (FITC) Mouse Monoclonal FCM 10001-MM08-F
    Anti-EGFR Antibody Rabbit Monoclonal Neutralization 10001-REN01
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