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EGFR / HER1 / ErbB1 Antibody, Rabbit MAb

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Human EGFR/ErbB1/HER1 Antibody Product Information
Immunogen:Recombinant Human EGFR / HER1 / ErbB1 protein (Catalog#10001-H08H)
Clone ID:043
Ig Type:Rabbit IgG
Concentration:
Endotoxin:
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:This antibody was obtained from a rabbit immunized with purified, recombinant Human EGFR / HER1 / ErbB1 (rh EGFR / HER1 / ErbB1; Catalog#10001-H08H; NP_005219; Met 1-Ser 645).
Human EGFR/ErbB1/HER1 Antibody Usage Guide
Specificity:Human EGFR / HER1 / ErbB1
No cross-reactivity in ELISA with
Human ErbB2
Human ErbB3
Application:ELISA, IHC-P, IHC-Fr, IF

ELISA: 0.1-0.2 μg/mL

This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human EGFR.

IHC-P: 1-10 μg/mL

IHC-Fr: 20-50 μg/mL

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Human EGFR/ErbB1/HER1 Antibody IHC Application Image
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Caption:
Immunofluorescence staining of EGFR in monkey skin with rabbit monoclonal antibody (20 μg/mL, frozen section). The image showing membrane staining of squamous epithelium cell.
EGFR / HER1 / ErbB1 Antibody, Rabbit MAb, Immunofluorescence
[Click to enlarge image]
Caption:
Immunochemical staining of human EGFR in human placenta with rabbit monoclonal antibody (5 µg/mL,formalin-fixed paraffin embedded sections). Positive staining was localized to trophoblast.
EGFR / HER1 / ErbB1 Antibody, Rabbit MAb, Immunohistochemistry
[Click to enlarge image]
Caption:
Immunochemical staining of human EGFR in human rectal cancer with rabbit monoclonal antibody (5 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to rectal gland. The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).
EGFR / HER1 / ErbB1 Antibody, Rabbit MAb, Immunohistochemistry
[Click to enlarge image]
Caption:
Immunochemical staining of human EGFR in human uterine cervix cancer with rabbit monoclonal antibody (5 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to uterine gland.
EGFR / HER1 / ErbB1 Antibody, Rabbit MAb, Immunohistochemistry
Other EGFR/ErbB1/HER1 Antibody Products
EGFR/HER1/ErbB1 Background

As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.

Human EGFR/HER1/ErbB1 References
  • Schlessinger, J. (2000) Cell signaling by receptor tyrosine kinases. Cell 103(2): 211-25.
  • Giaccone, G. (2005) HER1/EGFR-targeted agents: predicting the future for patients with unpredictable outcomes to therapy. Ann. Oncol. 16(4): 538-48.
  • Yarden, Y., et al. (2001) Untangling the ErbB signalling network. Nat. Rev. Mol. Cell. Biol. 2(2): 127-37.
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    Catalog: 10001-R043-50
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    All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"