EGFR / HER1 / ErbB1 Antibody

EGFR / HER1 / ErbB1 Antibody Datasheet

EGFR / HER1 / ErbB1 Antibody Product Information

• Immunogen: Recombinant Human EGFR / HER1 / ErbB1 protein (Catalog#10001-H08H)
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 043
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Human EGFR / HER1 / ErbB1 (rh EGFR / HER1 / ErbB1; Catalog#10001-H08H; NP_005219; Met 1-Ser 645).

EGFR / HER1 / ErbB1 Antibody Usage Guide

• Specificity: Human EGFR / HER1 / ErbB1
• No cross-reactivity in ELISA with
  

  Human ErbB2

  Human ErbB3

• Immunochemistry: IHC-P: 1-10 μg/mL

  IHC-Fr: 20-50 μg/mL

  

  Immunochemical staining of human EGFR in human placenta with rabbit monoclonal antibody (5 μg/mL,formalin-fixed paraffin embedded sections). Positive staining was localized to trophoblast.

  Immunochemical staining of human EGFR in human uterine cervix cancer with rabbit monoclonal antibody (5 μg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to uterine gland.

  

  Immunochemical staining of human EGFR in human rectal cancer with rabbit monoclonal antibody (5 μg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to rectal gland. The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).

  

  Immunofluorescence staining of EGFR in monkey skin with rabbit monoclonal antibody (20 μg/mL, frozen section). The image showing membrane staining of squamous epithelium cell.

• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human EGFR in WB.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human EGFR.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

EGFR / HER1 / ErbB1 Antibody Background

As a member of the epidermal growth factor receptor tyrosine kinases subfamily, EGF R is a type Ⅰ transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR signaling plays a crucial role in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR homo- or hetero- dimerization, initiates subsequent tyrosine autophosphorylation and various downstream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling has been shown to exert actions on carcinogenesis and disease progression, and thus EGFR was proposed as a target for cancer therapy currently.

References

1. Schlessinger, J., 2000, Cell. 103:211-225.
2. Giaccone, G., 2005, Ann. Onc. 16: 538-548.
3. Yarden, Y., 2001, Nat. Rev. Mol. Cell. Biol. 2: 127-137.