EGFR / HER1 / ErbB1 Antibody

EGFR / ErbB / HER1 Antibody Datasheet

EGFR / ErbB / HER1 Antibody Product Information

• Immunogen: Recombinant Human EGFR / ErbB / HER1 Protein (Catalog #10001-H08H)
• Antibody Type: Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
• Clone ID: 3D2H11C5
• Ig Type: Mouse IgG2b
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, human cell-derived, recombinant Human EGFR / ErbB / HER1 (rHuman EGFR / ErbB / HER1; Catalog #10001-H08H; Met 1-Ser 645; NP_005219). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

EGFR / ErbB / HER1 Antibody Usage Guide

• Specificity: Human EGFR / ErbB / HER1
• No cross-reactivity in ELISA with
  

  Human EGFR/ErbB1

  Human ErbB2

  Human ErbB3

  Human ErbB4

  Human cell lysate (293 cell line)

• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human EGFR in WB. Using a DAB detection system, the detection limit for Human EGFR is approximately 1 ng/lane under non-reducing conditions. Use of this antibody under reducing conditions is not recommended.
• Direct ELISA: This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human EGFR. The detection limit for Human EGFR is approximately 0.0195 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

EGFR / ErbB / HER1 Antibody Background

As a member of the epidermal growth factor receptor tyrosine kinases subfamily, EGF R is a type Ⅰ transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR signaling plays a crucial role in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR homo- or hetero- dimerization, initiates subsequent tyrosine autophosphorylation and various downstream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling has been shown to exert actions on carcinogenesis and disease progression, and thus EGFR was proposed as a target for cancer therapy currently.

References

1. Schlessinger, J., 2000, Cell. 103:211-225.
2. Giaccone, G., 2005, Ann. Onc. 16: 538-548.
3. Yarden, Y., 2001, Nat. Rev. Mol. Cell. Biol. 2: 127-137.