|Recombinant Human EGFR / ErbB / HER1 protein (Catalog#10001-H08H)|
|0.2 μm filtered solution in PBS|
|Produced in rabbits immunized with purified, recombinant Human EGFR / ErbB / HER1 (rh EGFR / ErbB / HER1; Catalog#10001-H08H; Met 1-Ser 645; NP_005219). Total IgG was purified by Protein A affinity chromatography.|
This antibody can be used at 1:1000-1:2000 with the appropriate secondary reagents to detect Human EGFR / HER1 / ErbB1.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.