Anti-EGFR Antibody

Cat: 10001-R021
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Anti-EGFR Antibody (Rabbit Monoclonal antibody) General Information
Product name
Anti-EGFR Antibody
Validated applications
WB,ELISA,ELISA(Det),ICC/IF,IF
Species reactivity
Reacts with: Human
Specificity
Human EGFR
No cross-reactivity in ELISA with Human ErbB2 / HER2 Human ErbB3 / HER3
Immunogen
Recombinant Human EGFR Protein(Catalog#10001-H08H)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human EGFR (rh EGFR; Catalog#10001-H08H; Met 1-Ser 645; NP_005219) .
Source
Monoclonal Rabbit IgG Clone #021
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-EGFR Antibody (Rabbit Monoclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:1000  
ELISA 1:25000-1:50000  
ICC-IF 1:100-1:500  
ELISA(Det) 1:5000-1:50000 This antibody will detect Human EGFR / HER1 in ELISA pair set (Catalog: # SEK10001). In a sandwich ELISA, it can be used as detection antibody when paired with (Catalog: # 10001-RE11).

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-EGFR Antibody (Rabbit Monoclonal antibody) Images
Human EGFR/HER1 Immunoprecipitation(IP) 7922

EGFR was immunoprecipitated using:

Lane A:0.5 mg A431 Whole Cell Lysate

2 µL anti-EGFR rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-EGFR rabbit monoclonal antibody,at 1:100 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 134 kDa

Observed band size: 134 kDa

Human EGFR/HER1 Western blot (WB) 13024

Anti-EGFR rabbit monoclonal antibody at 1:500 dilution

Lane A: A431 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:180 kDa

Observed band size:180 kDa

Human EGFR/HER1 Immunofluorescence(IF) 14800
Immunofluorescence staining of Human EGFR in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human EGFR monoclonal antibody (1:300) at 4℃ overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).
Anti-EGFR Antibody Alternative Names
Anti-ERBB Antibody;Anti-ERBB1 Antibody;Anti-HER1 Antibody;Anti-mENA Antibody;Anti-NISBD2 Antibody;Anti-PIG61 Antibody
EGFR Background Information

As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.

Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Full Name
epidermal growth factor receptor
References
  • Schlessinger, J. (2000) Cell signaling by receptor tyrosine kinases. Cell 103(2): 211-25.
  • Giaccone, G. (2005) HER1/EGFR-targeted agents: predicting the future for patients with unpredictable outcomes to therapy. Ann. Oncol. 16(4): 538-48.
  • Yarden, Y., et al. (2001) Untangling the ErbB signalling network. Nat. Rev. Mol. Cell. Biol. 2(2): 127-37.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-EGFR Antibody Rabbit Polyclonal ELISA 90317-T16
    Anti-EGFR Antibody Rabbit Polyclonal ELISA 90317-RP01
    Anti-EGFR Antibody Rabbit Polyclonal WB,ELISA,IP 80100-T52
    Anti-EGFR Antibody Rabbit Polyclonal ELISA 80100-RP01
    Anti-EGFR Antibody Rabbit Polyclonal WB,ELISA,IP 51091-T52
    Anti-EGFR Antibody Rabbit Monoclonal ELISA 51091-R112
    Anti-EGFR Antibody Rabbit Polyclonal WB,ELISA 51091-T48
    Anti-EGFR Antibody Rabbit Monoclonal ELISA 51091-R004
    Anti-EGFR Antibody Rabbit Polyclonal ELISA 51091-RP01
    Anti-EGFR Antibody Rabbit Polyclonal ELISA 10001-RP02
    Anti-EGFR Antibody Mouse Monoclonal ELISA 10001-MM01
    Anti-EGFR Antibody (APC) Mouse Monoclonal FCM 10001-MM08-A
    Anti-EGFR Antibody (APC) Rabbit Monoclonal FCM 10001-RE01-A
    Anti-EGFR Antibody Rabbit Polyclonal ELISA,IHC-P 10001-T24
    Anti-EGFR Antibody Rabbit Polyclonal ELISA 10001-RP01
    Anti-EGFR Antibody Rabbit Polyclonal WB 100372-T32
    Anti-EGFR Antibody Rabbit Monoclonal Neutralization 10001-RE10
    Anti-EGFR Antibody (PE) Mouse Monoclonal FCM 10001-MM08-P
    Anti-EGFR Antibody Rabbit Monoclonal ELISA,FCM 10001-RE01
    Anti-EGFR Antibody Rabbit Monoclonal ELISA,IHC-P,IHC-Fr 10001-R043
    Anti-EGFR Antibody Mouse Monoclonal FCM,ICC/IF,IF 10001-MM08
    Anti-EGFR Antibody Mouse Monoclonal IHC-P 10001-MM11
    Anti-EGFR Antibody Rabbit Monoclonal ELISA(Cap) 10001-RE11
    Anti-EGFR Antibody (PE) Rabbit Monoclonal FCM 10001-RE01-P
    Anti-EGFR Antibody (FITC) Rabbit Monoclonal FCM 10001-RE01-F
    Anti-EGFR Antibody (FITC) Mouse Monoclonal FCM 10001-MM08-F
    Anti-EGFR Antibody Rabbit Monoclonal Neutralization 10001-REN01
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