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EGFR/HER1 Mouse MAb

  • EGFR / HER1 / ErbB1 Antibody, Mouse MAb, Flow cytometric analysis
  • EGFR / HER1 / ErbB1 Antibody, Mouse MAb, Immunofluorescence
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Human EGFR Antibody Product Information
Immunogen:Recombinant Human EGFR / HER1 / ErbB1 protein (Catalog#10001-H08H)
Clone ID:08
Ig Type:Mouse IgG1
Concentration:
Endotoxin:
Formulation:0.2 μm filtered solution in PBS
Preparation:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human EGFR / HER1 / ErbB1 (rh EGFR / HER1 / ErbB1; Catalog#10001-H08H; NP_005219; Met1-Ser645). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Human EGFR Antibody IF Application Image
EGFR / HER1 / ErbB1 Antibody, Mouse MAb, Immunofluorescence
[Click to enlarge image]
Caption:
Immunofluorescence staining of Human EGFR in A431 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Mouse anti-Human EGFR monoclonal antibody (10 µg/ml) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated (left panel, captured by laser confocal scanning microscope) and Alexa Fluor®594-conjugated (right panel, captured by fluorescence microscope) Goat Anti-mouse IgG secondary antibody, countstained with DAPI (blue). Positive staining was localized to plasma membrane.
Human EGFR Antibody FC Application Image
EGFR / HER1 / ErbB1 Antibody, Mouse MAb, Flow cytometric analysis
[Click to enlarge image]
Caption:
Flow cytometric analysis of Human EGFR expression on A431 cells. Cells were stained with purified anti-Human EGFR, then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Other EGFR Antibody Products
Reactivity: Human  
Application: ELISA  IHC-P  
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10001-T24-200
10001-T24-50
10001-T24-100
200 µg 
50 µg 
100 µg 
Add to Cart
Reactivity: Human  
Application: B/N  
  • Slide 1
10001-RE10-200
10001-RE10-500
200 µg 
500 µg 
Add to Cart
Reactivity: Human  
Application: ELISA  
    10001-RP02-50
    10001-RP02-100
    10001-RP02-200
    50 µg 
    100 µg 
    200 µg 
    Add to Cart
    Reactivity: Human  
    Application: B/N  
    • Slide 1
    10001-REN01-200
    10001-REN01-500
    200 µg 
    500 µg 
    Add to Cart
    Reactivity: Human  
    Application: ELISA  WB  IP  
    • Slide 1
    • Slide 1
    10001-R021-50
    10001-R021-100
    50 µg 
    100 µg 
    Add to Cart
    Reactivity: Human  
    Application: ELISA  
      10001-RP01-400
      10001-RP01-100
      10001-RP01-200
       
      100 µg 
      200 µg 
      Add to Cart
      Reactivity: Human  
      Application: ELISA  
        10001-MM01-200
        10001-MM01-50
        10001-MM01-100
        200 µg 
        50 µg 
        100 µg 
        Add to Cart
        Reactivity: Human  
        Application: ELISA  IHC-P  IF  IHC-Fr  
        • Slide 1
        • Slide 1
        • Slide 1
        • Slide 1
        10001-R043-50
        10001-R043-100
        50 µg 
        100 µg 
        Add to Cart
        Reactivity: Human  
        Application: FCM  
        • Slide 1
        10001-RE01-F-50
        10001-RE01-F-100
        50 Tests 
        100 Tests 
        Add to Cart
        Reactivity: Human  
        Application: FCM  
        • Slide 1
        10001-MM08-F-50
        10001-MM08-F-100
        50 Tests 
        100 Tests 
        Add to Cart
        Reactivity: Human  
        Application: FCM  
        • Slide 1
        10001-MM08-P-50
        10001-MM08-P-100
        50 Tests 
        100 Tests 
        Add to Cart
        Reactivity: Human  
        Application: FCM  
        • Slide 1
        10001-RE01-P-50
        10001-RE01-P-100
        50 Tests 
        100 Tests 
        Add to Cart
        Reactivity: Human  
        Application: ELISA  
          10001-RE01-100
          10001-RE01-50
          100 µg 
          50 µg 
          Add to Cart
          Reactivity: Human  
          Application: WB  
          • Slide 1
          100372-T32-50
          100372-T32-100
          100372-T32-200
          50 µg 
          100 µg 
          200 µg 
          Add to Cart
          Reactivity: Human  
          Application: FCM  
          • Slide 1
          10001-RE01-A-100
          10001-RE01-A-50
          100 Tests 
          50 Tests 
          Add to Cart
          Reactivity: Human  
          Application: FCM  
          • Slide 1
          10001-MM08-A-100
          10001-MM08-A-50
          100 Tests 
          50 Tests 
          Add to Cart
          Reactivity: Human  
          Application: IHC-P  
          • Slide 1
          • Slide 1
          • Slide 1
          • Slide 1
          • Slide 1
          10001-MM11-100
          10001-MM11-50
          10001-MM11-200
          100 µg 
          50 µg 
          200 µg 
          Add to Cart
          Reactivity: Mouse  
          Application: ELISA  
            51091-R112-50
            51091-R112-100
            50 µg 
            100 µg 
            Add to Cart
            Reactivity: Mouse  
            Application: ELISA  
              51091-R004-50
              51091-R004-100
              50 µg 
              100 µg 
              Add to Cart
              Reactivity: Mouse  
              Application: ELISA  WB  IP  
              • Slide 1
              • Slide 1
              51091-T52-200
              51091-T52-50
              51091-T52-100
              200 µg 
              50 µg 
              100 µg 
              Add to Cart
              Reactivity: Mouse  
              Application: ELISA  
                51091-RP01-200
                51091-RP01-400
                51091-RP01-100
                200 µg 
                 
                100 µg 
                Add to Cart
                Reactivity: Rat  
                Application: ELISA  
                  80100-RP01-100
                  80100-RP01-200
                  100 µg 
                  200 µg 
                  Add to Cart
                  Reactivity: Rat  
                  Application: ELISA  WB  IP  
                  • Slide 1
                  • Slide 1
                  80100-T52-200
                  80100-T52-100
                  80100-T52-50
                  200 µg 
                  100 µg 
                  50 µg 
                  Add to Cart
                  Reactivity: Rhesus  
                  Application: ELISA  
                    90317-RP01-200
                    90317-RP01-100
                    90317-RP01-400
                    200 µg 
                    100 µg 
                     
                    Add to Cart
                    Reactivity: Rhesus  
                    Application: ELISA  
                      90317-T16-100
                      90317-T16-200
                      90317-T16-50
                      100 µg 
                      200 µg 
                      50 µg 
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                      EGFR/HER1 Background

                      As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.

                      Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

                      Human EGFR/HER1 References
                    • Schlessinger, J. (2000) Cell signaling by receptor tyrosine kinases. Cell 103(2): 211-25.
                    • Giaccone, G. (2005) HER1/EGFR-targeted agents: predicting the future for patients with unpredictable outcomes to therapy. Ann. Oncol. 16(4): 538-48.
                    • Yarden, Y., et al. (2001) Untangling the ErbB signalling network. Nat. Rev. Mol. Cell. Biol. 2(2): 127-37.
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                      Size / Price
                      Catalog: 10001-MM08-50
                      List Price: 
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                      Datasheet & Documentation

                      All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
                      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"