EGF Protein & Antibody

Epidermal Growth Factor

EGF Products

EGF Protein, Recombinant

Molecule	Species	Description	Cat. No
EGF	Human	EGF/Fc Protein, Recombinant	10605-H01H
EGF	Human	EGF Protein, Recombinant	10605-HNAE
EGF	Mouse	EGF Protein, Recombinant	50482-M01H
EGF	Canine	EGF / Epidermal Growth Factor Protein, Recombinant	70013-D07E

  10605-H01H: ED₅₀ = 47 pg / mL Measured in a cell proliferation assay using BALB/c 3T3 mouse embryonic fibroblasts.

  10605-HNAE: The ED₅₀ for this effect is typically 20-100 pg/mL. Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblasts. (Rubin, J.S. et al. 1991, Proc. Natl. Acad. Sci. USA 88:415.).

EGF Antibody

Molecule	Application	Description	Cat. No
Human EGF	WB, ELISA	EGF Antibody, Rabbit MAb	10605-R008

EGF cDNA Clone

Molecule	Species	Description	Cat. No
EGF	Mouse	Mus musculus EGF cDNA Clone	MG50482-M

EGF Related Areas

Cancer>>Cancer Biomarkers>>EGF

Cancer>>Growth Factor & Receptor>>EGF Family & Receptor>>EGF

EGF Alternative Names

EGF, HOMG4, URG [Homo sapiens]

Egf, AI790464 [Mus musculus]

EGF Related Products

Protein Products

Molecule	Species	Description
EGFR/ HER1/ErbB1	Human	EGFR/HER1/ErbB1/Fc Protein, Recombinant
EGFR/ HER1/ErbB1	Human	EGFR/HER1/ErbB1 Protein, Recombinant
HER2/ErbB2	Human	HER2/ErbB2/Fc Protein, Recombinant
HER2/ErbB2	Human	HER2/ErbB2 Protein, Recombinant
HER3/ErbB3	Human	HER3/ErbB3/Fc Protein, Recombinant
HER4/ErbB4	Human	HER4/ErbB4/Fc Protein, Recombinant

Antibody Products

Molecule	Application	Description
Human HER2/ErbB2	ELISA	Mouse Monoclonal antibody
Human HER2/ErbB2	WB	Mouse Monoclonal Antibody
Human HER2/ErbB2	ELISA	Rabbit Polyclonal Antibody

cDNA Clone Products

Molecule	Species	Description
Amphiregulin/AREG	Human	Homo sapiens Amphiregulin /AREG cDNA Clone(NM_001657.2)
EGF-L6	Human	Homo sapiens EGF-L6 cDNA Clone(NM_015507.2)
HB-EGF	Human	Homo sapiens HB-EGF cDNA Clone(NM_001945.2)
HER2/ErbB2	Human	Homo sapiens HER2/ErbB2 transcript variant 1 cDNA Clone(NM_004448.2)
HER3/ErbB3	Human	Homo sapiens HER3/ErbB3 transcript variant 1 cDNA Clone(NM_001982.2)
HER4/ErbB4	Human	Homo sapiens HER4/ErbB4 transcript variant JM-a/CVT-2 cDNA Clone(NM_001042599.1)

EGF Background

EGF is the founding member of the EGF-family of proteins. Members of this protein family have highly similar structural and functional characteristics. EGF contains 9 EGF-like domains and 9 LDL-receptor class B repeats. Human EGF is a 6045-Da protein with 53 amino acid residues and three intramolecular disulfide bonds. As a low-molecular-weight polypeptide, EGF was first purified from the mouse submandibular gland, but since then it was found in many human tissues including submandibular gland, parotid gland. It can also be found in human platelets, macrophages, urine, saliva, milk, and plasma. EGF is a growth factor that stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. It results in cellular proliferation, differentiation, and survival. Salivary EGF, which seems also regulated by dietary inorganic iodine, also plays an important physiological role in the maintenance of oro-esophageal and gastric tissue integrity. EGF acts by binding with high affinity to epidermal growth factor receptor on the cell surface and stimulating the intrinsic protein-tyrosine kinase activity of the receptor. The tyrosine kinase activity, in turn, initiates a signal transduction cascade that results in a variety of biochemical changes within the cell - a rise in intracellular calcium levels, increased glycolysis and protein synthesis, and increases in the expression of certain genes including the gene for EGFR - that ultimately lead to DNA synthesis and cell proliferation.

EGF Related Studies

1. Chen JX, et al. (2011) Involvement of c-Src/STAT3 signal in EGF-induced proliferation of rat spermatogonial stem cells. Mol Cell Biochem. 358(1-2):67-73.
2. Guo Y, et al. (2012) Correlations among ERCC1, XPB, UBE2I, EGF, TAL2 and ILF3 revealed by gene signatures of histological subtypes of patients with epithelial ovarian cancer. Oncol Rep. 27(1):286-92.
3. Kim S, et al. (2012) Smad7 acts as a negative regulator of the epidermal growth factor (EGF) signaling pathway in breast cancer cells. Cancer Lett. 314(2):147-54.
4. Chatterton RT Jr, et al. (2010) Breast ductal lavage for assessment of breast cancer biomarkers. Horm Cancer. 1(4):197-204.