Rat EFNB3 / Ephrin B3 Protein

Ephrin B3 Protein Datasheet

EFNB3 / Ephrin B3 Protein Product Information

• Synonym: EFNB3
• Protein Construction: A DNA sequence encoding the rat EFNB3 (NP_001094450) (Met1-Ser224) was expressed, fused with the Fc region of human IgG₁ at the C-terminus.
• Source: Rat
• Expression Host: Human Cells

EFNB3 / Ephrin B3 Protein QC Testing

• Purity: (79.6+17)% as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Leu 28
• Molecular Mass: The recombinant rat EFNB3/Fc is a disulfide-linked homodimer. The reduced monomer comprises 438 amino acids and has a predicted molecular mass of 48.7 kDa. The apparent molecular mass of the protein is approximately 55 and 34 kDa in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: EFNB3 / Ephrin B3 protein

EFNB3 / Ephrin B3 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

EFNB3 / Ephrin B3 Protein Description

Ephrin B3 is a member of the ephrin family. There are two types of ephrins: the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. Ephrin B3 is a cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Ephrin B3 is important in brain development as well as in its maintenance. It is especially important for forebrain function since its expression levels were particularly high in several forebrain subregions compared to other brain subregions.

References

1. Takemoto M. et al., 2002, Eur J Neurosci. 16 (6): 1168-72.
2. Brückner K. et al., 1999, Neuron. 22 (3): 511-24.
3. Bergemann A. et al., 1998, Oncogene. 16 (4): 471-80.