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Your Position: Home > Gene Products > Other Species > Danio rerio (zebrafish) EFNB2A Gene cDNA Clone / ORF Clone

Danio rerio (zebrafish) EFNB2A Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
ZG63002-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

EFNB2A cDNA Clone Datasheet

EFNB2A cDNA Clone Product Information

Gene Bank Ref.ID:	AF375225.1
cDNA Size:	999
Gene Synonym:	efnb2; ephrin-B2a; id:ibd5072; efnb2a
Source:	Danio rerio (zebrafish)

EFNB2A cDNA Clone Description

cDNA Description:	ORF Clone of Danio rerio (zebrafish) ephrin B2a DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

EFNB2A cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

EFNB2A cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

EFNB2A cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGCGACT CTTTGTGGAG ATATTACTTT GGAGTTTTAG TGATCGCGTG CAAGGTGAAC
61       CTGTCCCGCG CGCTCATCCT GGACTCCATA TACTGGAACA CCACGAACAC CAAGTTTGTG
121      CCAGGACAGG GTCTGGTGCT GTATCCGCAG ATTGGGGATA AGATGGACAT AGTTTGCCCA
181      CGTGTGGAAG GTGGTTCAAT GGAAGGAGTG GAGTATTATA AACTCTATAT GGTTCCTCTG
241      GAACAGCTAA AGTCCTGTCA GGTCACAAAA GCAGACACGC CTCTACTCAA CTGCGTCAAG
301      CCAGACCAGG ACGTCAAATT CACCCTCAAA TTCCAGGAGT TCAGCCCAAA CCTTTGGGGC
361      CTGGAGTTCT TTAGAGGGAA AGACTACTAC ATTATCTCTA CATCTAATGG AACAATGGAG
421      GGGCTGGATA ATCAGGAAGG AGGGGTGTGT AAAACCAAGT CGATGAAAAT CATCATGAAG
481      GTTGGACAAA ACCCCTCTGA TCCCATTTCC CCCAAAGACT ACCCTACCAG TTACCCTCCC
541      AAACACCCTG ACTTAGGGGG CAAGGACAGC AAATCGAATG AAGTACTTAA GCCAGATGCA
601      TCTCCTCATG GGGAAGATAA GGGAGATGGA AATAAATCCT CATCAGTCAT TGGCTCAGAG
661      GTGGCCCTGT TTGCCTGCAT CGCCTCAGCA AGCGTCATCG TCATCATCAT AATCATCATG
721      CTAGTTTTCC TTCTCCTGAA GTATCGACGA CGTCATCGCA AACACTCTCC TCAACACGCC
781      ACCACGTTGT CACTCAGCAC ATTAGCCACG CCCAAGAGGG GCGGCAGCGG CGGCAACAAC
841      AACGGCTCAG AGCCGAGCGA CATCATCATC CCACTGCGGA CTGCTGACAG CGTCTTCTGC
901      CCGCATTACG AGAAAGTGAG CGGCGATTAC GGACACCCTG TTTACATCGT ACAGGAAATG
961      CCGCCACAGA GCCCAGCAAA CATCTATTAC AAGGTGTGA

Quality Control:cDNA sequencing results is available upon request.