Human EDN3 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG12140-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

EDN3 cDNA Clone Datasheet

EDN3 cDNA Clone Product Information

Gene Bank Ref.ID:	BC008876
cDNA Size:	717
Gene Synonym:	ET3; ET-3; WS4B; HSCR4; PPET3; MGC15067; MGC61498;EDN3
Source:	Human

EDN3 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens endothelin 3 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

EDN3 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

EDN3 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

EDN3 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGAGCCGG GGCTGTGGCT CCTTTTCGGG CTCACAGTGA CCTCCGCCGC AGGATTCGTG
61       CCTTGCTCCC AGTCTGGGGA TGCTGGCAGG CGCGGCGTGT CCCAGGCCCC CACTGCAGCC
121      AGATCTGAGG GGGACTGTGA AGAGACTGTG GCTGGCCCTG GCGAGGAGAC TGTGGCTGGC
181      CCTGGCGAGG GGACTGTGGC CCCGACAGCA CTGCAGGGTC CAAGCCCTGG AAGCCCTGGG
241      CAGGAGCAGG CGGCCGAGGG GGCCCCTGAG CACCACCGAT CCAGGCGCTG CACGTGCTTC
301      ACCTACAAGG ACAAGGAGTG TGTCTACTAT TGCCACCTGG ACATCATTTG GATCAACACT
361      CCCGAACAGA CGGTGCCCTA TGGACTGTCC AACTACAGAG GAAGCTTCCG GGGCAAGAGG
421      TCTGCGGGGC CACTTCCAGG GAATCTGCAG CTCTCACATC GGCCACACTT GCGCTGCGCT
481      TGTGTGGGGA GATATGACAA GGCCTGCCTG CACTTTTGCA CCCAAACTCT GGACGTCAGC
541      AGTAATTCAA GGACGGCAGA AAAAACAGAC AAAGAAGAGG AAGGGAAGGT TGAAGTCAAG
601      GACCAACAAA GCAAGCAGGC TTTAGACCTC CACCATCCAA AGCTCATGCC CGGCAGTGGA
661      CTCGCCCTCG CTCCATCTAC CTGCCCCCGC TGCCTCTTTC AGGAAGGAGC CCCTTAG

Quality Control:cDNA sequencing results is available upon request.