Human EDN2 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13453-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

EDN2 cDNA Clone Datasheet

EDN2 cDNA Clone Product Information

Gene Bank Ref.ID:	BC034393
cDNA Size:	537
Gene Synonym:	ET2; PPET2; EDN2
Source:	Human

EDN2 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens endothelin 2 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

EDN2 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

EDN2 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

EDN2 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGTCTCCG TGCCTACCAC CTGGTGCTCC GTTGCGCTAG CCCTGCTCGT GGCCCTGCAT
61       GAAGGGAAGG GCCAGGCTGC TGCCACCCTG GAGCAGCCAG CGTCCTCATC TCATGCCCAA
121      GGCACCCACC TTCGGCTTCG CCGTTGCTCC TGCAGCTCCT GGCTCGACAA GGAGTGCGTC
181      TACTTCTGCC ACTTGGACAT CATCTGGGTG AACACTCCTG AACAGACAGC TCCTTACGGC
241      CTGGGAAACC CGCCAAGACG CCGGCGCCGC TCCCTGCCAA GGCGCTGTCA GTGCTCCAGT
301      GCCAGGGACC CCGCCTGTGC CACCTTCTGC CTTCGAAGGC CCTGGACTGA AGCCGGGGCA
361      GTCCCAAGCC GGAAGTCCCC TGCAGACGTG TTCCAGACTG GCAAGACAGG GGCCACTACA
421      GGAGAGCTTC TCCAAAGGCT GAGGGACATT TCCACAGTCA AGAGCCTCTT TGCCAAGCGA
481      CAACAGGAGG CCATGCGGGA GCCTCGGTCC ACACATTCCA GGTGGAGGAA GAGATAG

Quality Control:cDNA sequencing results is available upon request.