Human EDN1 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13459-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

EDN1 cDNA Clone Datasheet

EDN1 cDNA Clone Product Information

Gene Bank Ref.ID:	BC009720
cDNA Size:	639
Gene Synonym:	ET1; PPET1; HDLCQ7; EDN1
Source:	Human

EDN1 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens endothelin 1 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

EDN1 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

EDN1 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

EDN1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGATTATT TGCTCATGAT TTTCTCTCTG CTGTTTGTGG CTTGCCAAGG AGCTCCAGAA
61       ACAGCAGTCT TAGGCGCTGA GCTCAGCGCG GTGGGTGAGA ACGGCGGGGA GAAACCCACT
121      CCCAGTCCAC CCTGGCGGCT CCGCCGGTCC AAGCGCTGCT CCTGCTCGTC CCTGATGGAT
181      AAAGAGTGTG TCTACTTCTG CCACCTGGAC ATCATTTGGG TCAACACTCC CGAGCACGTT
241      GTTCCGTATG GACTTGGAAG CCCTAGGTCC AAGAGAGCCT TGGAGAATTT ACTTCCCACA
301      AAGGCAACAG ACCGTGAGAA TAGATGCCAA TGTGCTAGCC AAAAAGACAA GAAGTGCTGG
361      AATTTTTGCC AAGCAGGAAA AGAACTCAGG GCTGAAGACA TTATGGAGAA AGACTGGAAT
421      AATCATAAGA AAGGAAAAGA CTGTTCCAAG CTTGGGAAAA AGTGTATTTA TCAGCAGTTA
481      GTGAGAGGAA GAAAAATCAG AAGAAGTTCA GAGGAACACC TAAGACAAAC CAGGTCGGAG
541      ACCATGAGAA ACAGCGTCAA ATCATCTTTT CATGATCCCA AGCTGAAAGG CAATCCCTCC
601      AGAGAGCGTT ATGTGACCCA CAACCGAGCA CATTGGTGA

Quality Control:cDNA sequencing results is available upon request.